TY - JOUR
T1 - Targets of gene amplification and overexpression at 17q in gastric cancer
AU - Varis, Asta
AU - Wolf, Maija
AU - Monni, Outi
AU - Vakkari, Marja Leena
AU - Kokkola, Arto
AU - Moskaluk, Chris
AU - Frierson, Henry
AU - Powell, Steven M.
AU - Knuutila, Sakari
AU - Kallioniemi, Anne
AU - El-Rifai, Wa’el
PY - 2002/5/1
Y1 - 2002/5/1
N2 - DNA copy number gains and amplifications at 17q are frequent in gastric cancer, yet systematic analyses of the 17q amplicon have not been performed. In this study, we carried out a comprehensive analysis of copy number and expression levels of 636 chromosome 17-specific genes in gastric cancer by using a custom-made chromosome 17-specific cDNA microarray. Analysis of DNA copy number changes by comparative genomic hybridization on cDNA microarray revealed increased copy numbers of 11 known genes (ERBB2, TOP2A, GRB7, ACLY, P1P5K2B, MPRL45, MKP-L, LHX1, MLN51, MLN64, and RPL27) and seven expressed sequence tags (ESTs) that mapped to 17q12-q21 region. To investigate the genes transcribed at the 17q, we performed gene expression analyses on an identical cDNA microarray. Our expression analysis showed overexpression of 8 genes (ERBB2, TOP2A, GRB2, AOC3, AP2B1, KRT14, JUP, and ITGA3) and two ESTs. Of the commonly amplified transcripts, an uncharacterized EST AA552509 and the TOP2A gene were most frequently overexpressed in 82% of the samples. Additional studies will be initiated to understand the possible biological and clinical significance of these genes in gastric cancer development and progression.
AB - DNA copy number gains and amplifications at 17q are frequent in gastric cancer, yet systematic analyses of the 17q amplicon have not been performed. In this study, we carried out a comprehensive analysis of copy number and expression levels of 636 chromosome 17-specific genes in gastric cancer by using a custom-made chromosome 17-specific cDNA microarray. Analysis of DNA copy number changes by comparative genomic hybridization on cDNA microarray revealed increased copy numbers of 11 known genes (ERBB2, TOP2A, GRB7, ACLY, P1P5K2B, MPRL45, MKP-L, LHX1, MLN51, MLN64, and RPL27) and seven expressed sequence tags (ESTs) that mapped to 17q12-q21 region. To investigate the genes transcribed at the 17q, we performed gene expression analyses on an identical cDNA microarray. Our expression analysis showed overexpression of 8 genes (ERBB2, TOP2A, GRB2, AOC3, AP2B1, KRT14, JUP, and ITGA3) and two ESTs. Of the commonly amplified transcripts, an uncharacterized EST AA552509 and the TOP2A gene were most frequently overexpressed in 82% of the samples. Additional studies will be initiated to understand the possible biological and clinical significance of these genes in gastric cancer development and progression.
UR - http://www.scopus.com/inward/record.url?scp=0036570356&partnerID=8YFLogxK
M3 - Article
C2 - 11980659
AN - SCOPUS:0036570356
SN - 0008-5472
VL - 62
SP - 2625
EP - 2629
JO - Cancer Research
JF - Cancer Research
IS - 9
ER -