Temperature Influences the Interaction between SARS-CoV-2 Spike from Omicron Subvariants and Human ACE2

Shang Yu Gong; Shilei Ding; Mehdi Benlarbi; Yaozong Chen; Dani Vézina; Lorie Marchitto; Guillaume Beaudoin-Bussières; Guillaume Goyette; Catherine Bourassa; Yuxia Bo; Halima Medjahed; Inès Levade; Marzena Pazgier; Marceline Côté; Jonathan Richard; Jérémie Prévost; Andrés Finzi

doi:10.3390/v14102178

Temperature Influences the Interaction between SARS-CoV-2 Spike from Omicron Subvariants and Human ACE2

Shang Yu Gong, Shilei Ding, Mehdi Benlarbi, Yaozong Chen, Dani Vézina, Lorie Marchitto, Guillaume Beaudoin-Bussières, Guillaume Goyette, Catherine Bourassa, Yuxia Bo, Halima Medjahed, Inès Levade, Marzena Pazgier, Marceline Côté, Jonathan Richard, Jérémie Prévost, Andrés Finzi^*

^*Corresponding author for this work

Medicine

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

SARS-CoV-2 continues to infect millions of people worldwide. The subvariants arising from the variant-of-concern (VOC) Omicron include BA.1, BA.1.1, BA.2, BA.2.12.1, BA.4, and BA.5. All possess multiple mutations in their Spike glycoprotein, notably in its immunogenic receptor-binding domain (RBD), and present enhanced viral transmission. The highly mutated Spike glycoproteins from these subvariants present different degrees of resistance to recognition and cross-neutralisation by plasma from previously infected and/or vaccinated individuals. We have recently shown that the temperature affects the interaction between the Spike and its receptor, the angiotensin converting enzyme 2 (ACE2). The affinity of RBD for ACE2 is significantly increased at lower temperatures. However, whether this is also observed with the Spike of Omicron and sub-lineages is not known. Here we show that, similar to other variants, Spikes from Omicron sub-lineages bind better the ACE2 receptor at lower temperatures. Whether this translates into enhanced transmission during the fall and winter seasons remains to be determined.

Original language	English
Article number	2178
Journal	Viruses
Volume	14
Issue number	10
DOIs	https://doi.org/10.3390/v14102178
State	Published - 30 Sep 2022

Keywords

Angiotensin-Converting Enzyme 2
COVID-19
Humans
Mutation
Peptidyl-Dipeptidase A/metabolism
SARS-CoV-2/genetics
Spike Glycoprotein, Coronavirus/metabolism
Temperature

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Gong, S. Y., Ding, S., Benlarbi, M., Chen, Y., Vézina, D., Marchitto, L., Beaudoin-Bussières, G., Goyette, G., Bourassa, C., Bo, Y., Medjahed, H., Levade, I., Pazgier, M., Côté, M., Richard, J., Prévost, J., & Finzi, A. (2022). Temperature Influences the Interaction between SARS-CoV-2 Spike from Omicron Subvariants and Human ACE2. Viruses, 14(10), Article 2178. https://doi.org/10.3390/v14102178

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title = "Temperature Influences the Interaction between SARS-CoV-2 Spike from Omicron Subvariants and Human ACE2",

abstract = "SARS-CoV-2 continues to infect millions of people worldwide. The subvariants arising from the variant-of-concern (VOC) Omicron include BA.1, BA.1.1, BA.2, BA.2.12.1, BA.4, and BA.5. All possess multiple mutations in their Spike glycoprotein, notably in its immunogenic receptor-binding domain (RBD), and present enhanced viral transmission. The highly mutated Spike glycoproteins from these subvariants present different degrees of resistance to recognition and cross-neutralisation by plasma from previously infected and/or vaccinated individuals. We have recently shown that the temperature affects the interaction between the Spike and its receptor, the angiotensin converting enzyme 2 (ACE2). The affinity of RBD for ACE2 is significantly increased at lower temperatures. However, whether this is also observed with the Spike of Omicron and sub-lineages is not known. Here we show that, similar to other variants, Spikes from Omicron sub-lineages bind better the ACE2 receptor at lower temperatures. Whether this translates into enhanced transmission during the fall and winter seasons remains to be determined.",

keywords = "Angiotensin-Converting Enzyme 2, COVID-19, Humans, Mutation, Peptidyl-Dipeptidase A/metabolism, SARS-CoV-2/genetics, Spike Glycoprotein, Coronavirus/metabolism, Temperature",

author = "Gong, {Shang Yu} and Shilei Ding and Mehdi Benlarbi and Yaozong Chen and Dani V{\'e}zina and Lorie Marchitto and Guillaume Beaudoin-Bussi{\`e}res and Guillaume Goyette and Catherine Bourassa and Yuxia Bo and Halima Medjahed and In{\`e}s Levade and Marzena Pazgier and Marceline C{\^o}t{\'e} and Jonathan Richard and J{\'e}r{\'e}mie Pr{\'e}vost and Andr{\'e}s Finzi",

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year = "2022",

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Gong, SY, Ding, S, Benlarbi, M, Chen, Y, Vézina, D, Marchitto, L, Beaudoin-Bussières, G, Goyette, G, Bourassa, C, Bo, Y, Medjahed, H, Levade, I, Pazgier, M, Côté, M, Richard, J, Prévost, J & Finzi, A 2022, 'Temperature Influences the Interaction between SARS-CoV-2 Spike from Omicron Subvariants and Human ACE2', Viruses, vol. 14, no. 10, 2178. https://doi.org/10.3390/v14102178

TY - JOUR

T1 - Temperature Influences the Interaction between SARS-CoV-2 Spike from Omicron Subvariants and Human ACE2

AU - Gong, Shang Yu

AU - Ding, Shilei

AU - Benlarbi, Mehdi

AU - Chen, Yaozong

AU - Vézina, Dani

AU - Marchitto, Lorie

AU - Beaudoin-Bussières, Guillaume

AU - Goyette, Guillaume

AU - Bourassa, Catherine

AU - Bo, Yuxia

AU - Medjahed, Halima

AU - Levade, Inès

AU - Pazgier, Marzena

AU - Côté, Marceline

AU - Richard, Jonathan

AU - Prévost, Jérémie

AU - Finzi, Andrés

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Y1 - 2022/9/30

N2 - SARS-CoV-2 continues to infect millions of people worldwide. The subvariants arising from the variant-of-concern (VOC) Omicron include BA.1, BA.1.1, BA.2, BA.2.12.1, BA.4, and BA.5. All possess multiple mutations in their Spike glycoprotein, notably in its immunogenic receptor-binding domain (RBD), and present enhanced viral transmission. The highly mutated Spike glycoproteins from these subvariants present different degrees of resistance to recognition and cross-neutralisation by plasma from previously infected and/or vaccinated individuals. We have recently shown that the temperature affects the interaction between the Spike and its receptor, the angiotensin converting enzyme 2 (ACE2). The affinity of RBD for ACE2 is significantly increased at lower temperatures. However, whether this is also observed with the Spike of Omicron and sub-lineages is not known. Here we show that, similar to other variants, Spikes from Omicron sub-lineages bind better the ACE2 receptor at lower temperatures. Whether this translates into enhanced transmission during the fall and winter seasons remains to be determined.

AB - SARS-CoV-2 continues to infect millions of people worldwide. The subvariants arising from the variant-of-concern (VOC) Omicron include BA.1, BA.1.1, BA.2, BA.2.12.1, BA.4, and BA.5. All possess multiple mutations in their Spike glycoprotein, notably in its immunogenic receptor-binding domain (RBD), and present enhanced viral transmission. The highly mutated Spike glycoproteins from these subvariants present different degrees of resistance to recognition and cross-neutralisation by plasma from previously infected and/or vaccinated individuals. We have recently shown that the temperature affects the interaction between the Spike and its receptor, the angiotensin converting enzyme 2 (ACE2). The affinity of RBD for ACE2 is significantly increased at lower temperatures. However, whether this is also observed with the Spike of Omicron and sub-lineages is not known. Here we show that, similar to other variants, Spikes from Omicron sub-lineages bind better the ACE2 receptor at lower temperatures. Whether this translates into enhanced transmission during the fall and winter seasons remains to be determined.

KW - Angiotensin-Converting Enzyme 2

KW - COVID-19

KW - Humans

KW - Mutation

KW - Peptidyl-Dipeptidase A/metabolism

KW - SARS-CoV-2/genetics

KW - Spike Glycoprotein, Coronavirus/metabolism

KW - Temperature

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U2 - 10.3390/v14102178

DO - 10.3390/v14102178

M3 - Article

C2 - 36298733

AN - SCOPUS:85140826065

SN - 1999-4915

VL - 14

JO - Viruses

JF - Viruses

IS - 10

M1 - 2178

ER -

Temperature Influences the Interaction between SARS-CoV-2 Spike from Omicron Subvariants and Human ACE2

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