Temporal Alterations in Cerebrovascular Glycocalyx and Cerebral Blood Flow after Exposure to a High-Intensity Blast in Rats

Ye Chen, Ming Gu, Jacob Patterson, Ruixuan Zhang, Jonathan K. Statz, Eileen Reed, Rania Abutarboush, Stephen T. Ahlers, Usmah Kawoos*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The glycocalyx is a proteoglycan–glycoprotein structure lining the luminal surface of the vascular endothelium and is susceptible to damage due to blast overpressure (BOP) exposure. The glycocalyx is essential in maintaining the structural and functional integrity of the vasculature and regulation of cerebral blood flow (CBF). Assessment of alterations in the density of the glycocalyx; its components (heparan sulphate proteoglycan (HSPG/syndecan-2), heparan sulphate (HS), and chondroitin sulphate (CS)); CBF; and the effect of hypercapnia on CBF was conducted at 2–3 h, 1, 3, 14, and 28 days after a high-intensity (18.9 PSI/131 kPa peak pressure, 10.95 ms duration, and 70.26 PSI·ms/484.42 kPa·ms impulse) BOP exposure in rats. A significant reduction in the density of the glycocalyx was observed 2–3 h, 1-, and 3 days after the blast exposure. The glycocalyx recovered by 28 days after exposure and was associated with an increase in HS (14 and 28 days) and in HSPG/syndecan-2 and CS (28 days) in the frontal cortex. In separate experiments, we observed significant decreases in CBF and a diminished response to hypercapnia at all time points with some recovery at 3 days. Given the role of the glycocalyx in regulating physiological function of the cerebral vasculature, damage to the glycocalyx after BOP exposure may result in the onset of pathogenesis and progression of cerebrovascular dysfunction leading to neuropathology.

Original languageEnglish
Article number3580
JournalInternational Journal of Molecular Sciences
Volume25
Issue number7
DOIs
StatePublished - Apr 2024
Externally publishedYes

Keywords

  • blast
  • cerebral blood flow
  • cerebrovascular glycocalyx
  • traumatic brain injury
  • vascular pathology

Fingerprint

Dive into the research topics of 'Temporal Alterations in Cerebrovascular Glycocalyx and Cerebral Blood Flow after Exposure to a High-Intensity Blast in Rats'. Together they form a unique fingerprint.

Cite this