The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33

Claire Corratgé-Faillie; Elsa Ronzier; Frédéric Sanchez; Karine Prado; Jeong Hyeon Kim; Sophie Lanciano; Nathalie Leonhardt; Benoît Lacombe; Tou Cheu Xiong

doi:10.1002/1873-3468.12687

The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33

Claire Corratgé-Faillie, Elsa Ronzier, Frédéric Sanchez, Karine Prado, Jeong Hyeon Kim, Sophie Lanciano, Nathalie Leonhardt, Benoît Lacombe, Tou Cheu Xiong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

A complex signaling network involving voltage-gated potassium channels from the Shaker family contributes to the regulation of stomatal aperture. Several kinases and phosphatases have been shown to be crucial for ABA-dependent regulation of the ion transporters. To date, the Ca²⁺-dependent regulation of Shaker channels by Ca²⁺-dependent protein kinases (CPKs) is still elusive. A functional screen in Xenopus oocytes was launched to identify such CPKs able to regulate the three main guard cell Shaker channels KAT1, KAT2, and GORK. Seven guard cell CPKs were tested and multiple CPK/Shaker couples were identified. Further work on CPK33 indicates that GORK activity is enhanced by CPK33 and unaffected by a nonfunctional CPK33 (CPK33-K102M). Furthermore, Ca²⁺-induced stomatal closure is impaired in two cpk33 mutant plants.

Original language	English
Pages (from-to)	1982-1992
Number of pages	11
Journal	FEBS Letters
Volume	591
Issue number	13
DOIs	https://doi.org/10.1002/1873-3468.12687
State	Published - 1 Jul 2017
Externally published	Yes

Keywords

CPKs
arabidopsis
guard cells
oocytes
shaker K channels
voltage clamp

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10.1002/1873-3468.12687

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title = "The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33",

abstract = "A complex signaling network involving voltage-gated potassium channels from the Shaker family contributes to the regulation of stomatal aperture. Several kinases and phosphatases have been shown to be crucial for ABA-dependent regulation of the ion transporters. To date, the Ca2+-dependent regulation of Shaker channels by Ca2+-dependent protein kinases (CPKs) is still elusive. A functional screen in Xenopus oocytes was launched to identify such CPKs able to regulate the three main guard cell Shaker channels KAT1, KAT2, and GORK. Seven guard cell CPKs were tested and multiple CPK/Shaker couples were identified. Further work on CPK33 indicates that GORK activity is enhanced by CPK33 and unaffected by a nonfunctional CPK33 (CPK33-K102M). Furthermore, Ca2+-induced stomatal closure is impaired in two cpk33 mutant plants.",

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AU - Corratgé-Faillie, Claire

AU - Ronzier, Elsa

AU - Sanchez, Frédéric

AU - Prado, Karine

AU - Kim, Jeong Hyeon

AU - Lanciano, Sophie

AU - Leonhardt, Nathalie

AU - Lacombe, Benoît

AU - Xiong, Tou Cheu

PY - 2017/7/1

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N2 - A complex signaling network involving voltage-gated potassium channels from the Shaker family contributes to the regulation of stomatal aperture. Several kinases and phosphatases have been shown to be crucial for ABA-dependent regulation of the ion transporters. To date, the Ca2+-dependent regulation of Shaker channels by Ca2+-dependent protein kinases (CPKs) is still elusive. A functional screen in Xenopus oocytes was launched to identify such CPKs able to regulate the three main guard cell Shaker channels KAT1, KAT2, and GORK. Seven guard cell CPKs were tested and multiple CPK/Shaker couples were identified. Further work on CPK33 indicates that GORK activity is enhanced by CPK33 and unaffected by a nonfunctional CPK33 (CPK33-K102M). Furthermore, Ca2+-induced stomatal closure is impaired in two cpk33 mutant plants.

AB - A complex signaling network involving voltage-gated potassium channels from the Shaker family contributes to the regulation of stomatal aperture. Several kinases and phosphatases have been shown to be crucial for ABA-dependent regulation of the ion transporters. To date, the Ca2+-dependent regulation of Shaker channels by Ca2+-dependent protein kinases (CPKs) is still elusive. A functional screen in Xenopus oocytes was launched to identify such CPKs able to regulate the three main guard cell Shaker channels KAT1, KAT2, and GORK. Seven guard cell CPKs were tested and multiple CPK/Shaker couples were identified. Further work on CPK33 indicates that GORK activity is enhanced by CPK33 and unaffected by a nonfunctional CPK33 (CPK33-K102M). Furthermore, Ca2+-induced stomatal closure is impaired in two cpk33 mutant plants.

KW - CPKs

KW - arabidopsis

KW - guard cells

KW - oocytes

KW - shaker K channels

KW - voltage clamp

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DO - 10.1002/1873-3468.12687

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AN - SCOPUS:85020298179

SN - 0014-5793

VL - 591

SP - 1982

EP - 1992

JO - FEBS Letters

JF - FEBS Letters

IS - 13

ER -

The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33

Abstract

Keywords

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