The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33

Claire Corratgé-Faillie, Elsa Ronzier, Frédéric Sanchez, Karine Prado, Jeong Hyeon Kim, Sophie Lanciano, Nathalie Leonhardt, Benoît Lacombe, Tou Cheu Xiong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


A complex signaling network involving voltage-gated potassium channels from the Shaker family contributes to the regulation of stomatal aperture. Several kinases and phosphatases have been shown to be crucial for ABA-dependent regulation of the ion transporters. To date, the Ca2+-dependent regulation of Shaker channels by Ca2+-dependent protein kinases (CPKs) is still elusive. A functional screen in Xenopus oocytes was launched to identify such CPKs able to regulate the three main guard cell Shaker channels KAT1, KAT2, and GORK. Seven guard cell CPKs were tested and multiple CPK/Shaker couples were identified. Further work on CPK33 indicates that GORK activity is enhanced by CPK33 and unaffected by a nonfunctional CPK33 (CPK33-K102M). Furthermore, Ca2+-induced stomatal closure is impaired in two cpk33 mutant plants.

Original languageEnglish
Pages (from-to)1982-1992
Number of pages11
JournalFEBS Letters
Issue number13
StatePublished - 1 Jul 2017
Externally publishedYes


  • CPKs
  • arabidopsis
  • guard cells
  • oocytes
  • shaker K channels
  • voltage clamp


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