The E2 'gene' of bovine papillomavirus encodes an enhancer-binding protein

C. Moskaluk; D. Bastia

doi:10.1073/pnas.84.5.1215

The E2 'gene' of bovine papillomavirus encodes an enhancer-binding protein

C. Moskaluk, D. Bastia

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

The E2 early open reading frame (presumably gene) of bovine papillomavirus-1 was fused in frame with the collagen-β-galactosidase-encoding region of the vector pJG200 and was expressed in and partially purified from Escherichia coli. The hybrid protein specifically bound to the enhancer region of bovine papillomavirus at several sites. DNase I-cleavage protection analysis of one such site revealed the protected sequence. A comparison of the protected sequence with the remainder of the DNA sequences that also have affinity for the protein revealed a consensus sequence having the motif AA/TTGGCGGNNC/G, in which N is any nucleotide. The protected region also includes a sequence with 2-fold rotational symmetry-ATCGGTG/CACCGAT.

Original language	English
Pages (from-to)	1215-1218
Number of pages	4
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	84
Issue number	5
DOIs	https://doi.org/10.1073/pnas.84.5.1215
State	Published - 1987
Externally published	Yes

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abstract = "The E2 early open reading frame (presumably gene) of bovine papillomavirus-1 was fused in frame with the collagen-β-galactosidase-encoding region of the vector pJG200 and was expressed in and partially purified from Escherichia coli. The hybrid protein specifically bound to the enhancer region of bovine papillomavirus at several sites. DNase I-cleavage protection analysis of one such site revealed the protected sequence. A comparison of the protected sequence with the remainder of the DNA sequences that also have affinity for the protein revealed a consensus sequence having the motif AA/TTGGCGGNNC/G, in which N is any nucleotide. The protected region also includes a sequence with 2-fold rotational symmetry-ATCGGTG/CACCGAT.",

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AB - The E2 early open reading frame (presumably gene) of bovine papillomavirus-1 was fused in frame with the collagen-β-galactosidase-encoding region of the vector pJG200 and was expressed in and partially purified from Escherichia coli. The hybrid protein specifically bound to the enhancer region of bovine papillomavirus at several sites. DNase I-cleavage protection analysis of one such site revealed the protected sequence. A comparison of the protected sequence with the remainder of the DNA sequences that also have affinity for the protein revealed a consensus sequence having the motif AA/TTGGCGGNNC/G, in which N is any nucleotide. The protected region also includes a sequence with 2-fold rotational symmetry-ATCGGTG/CACCGAT.

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The E2 'gene' of bovine papillomavirus encodes an enhancer-binding protein

Abstract

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