The Effect of Hematocrit on Platelet Adhesion: Experiments and Simulations

Andrew P. Spann, James E. Campbell, Sean R. Fitzgibbon, Armando Rodriguez, Andrew P. Cap, Lorne H. Blackbourne, Eric S.G. Shaqfeh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations

Abstract

The volume fraction of red blood cells (RBCs) in a capillary affects the degree to which platelets are promoted to marginate to near a vessel wall and form blood clots. In this work we investigate the relationship between RBC hematocrit and platelet adhesion activity. We perform experiments flowing blood samples through a microfluidic channel coated with type 1 collagen and observe the rate at which platelets adhere to the wall. We compare these results with three-dimensional boundary integral simulations of a suspension of RBCs and platelets in a periodic channel where platelets can adhere to the wall. In both cases, we find that the rate of platelet adhesion varies greatly with the RBC hematocrit. We observe that the relative decrease in platelet activity as hematocrit falls shows a similar profile for simulation and experiment.

Original languageEnglish
Pages (from-to)577-588
Number of pages12
JournalBiophysical Journal
Volume111
Issue number3
DOIs
StatePublished - 9 Aug 2016
Externally publishedYes

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