The Effect of Hematocrit on Platelet Adhesion: Experiments and Simulations

Andrew P. Spann; James E. Campbell; Sean R. Fitzgibbon; Armando Rodriguez; Andrew P. Cap; Lorne H. Blackbourne; Eric S.G. Shaqfeh

doi:10.1016/j.bpj.2016.06.024

The Effect of Hematocrit on Platelet Adhesion: Experiments and Simulations

Andrew P. Spann, James E. Campbell, Sean R. Fitzgibbon, Armando Rodriguez, Andrew P. Cap, Lorne H. Blackbourne, Eric S.G. Shaqfeh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

The volume fraction of red blood cells (RBCs) in a capillary affects the degree to which platelets are promoted to marginate to near a vessel wall and form blood clots. In this work we investigate the relationship between RBC hematocrit and platelet adhesion activity. We perform experiments flowing blood samples through a microfluidic channel coated with type 1 collagen and observe the rate at which platelets adhere to the wall. We compare these results with three-dimensional boundary integral simulations of a suspension of RBCs and platelets in a periodic channel where platelets can adhere to the wall. In both cases, we find that the rate of platelet adhesion varies greatly with the RBC hematocrit. We observe that the relative decrease in platelet activity as hematocrit falls shows a similar profile for simulation and experiment.

Original language	English
Pages (from-to)	577-588
Number of pages	12
Journal	Biophysical Journal
Volume	111
Issue number	3
DOIs	https://doi.org/10.1016/j.bpj.2016.06.024
State	Published - 9 Aug 2016
Externally published	Yes

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title = "The Effect of Hematocrit on Platelet Adhesion: Experiments and Simulations",

abstract = "The volume fraction of red blood cells (RBCs) in a capillary affects the degree to which platelets are promoted to marginate to near a vessel wall and form blood clots. In this work we investigate the relationship between RBC hematocrit and platelet adhesion activity. We perform experiments flowing blood samples through a microfluidic channel coated with type 1 collagen and observe the rate at which platelets adhere to the wall. We compare these results with three-dimensional boundary integral simulations of a suspension of RBCs and platelets in a periodic channel where platelets can adhere to the wall. In both cases, we find that the rate of platelet adhesion varies greatly with the RBC hematocrit. We observe that the relative decrease in platelet activity as hematocrit falls shows a similar profile for simulation and experiment.",

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AU - Spann, Andrew P.

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AU - Rodriguez, Armando

AU - Cap, Andrew P.

AU - Blackbourne, Lorne H.

AU - Shaqfeh, Eric S.G.

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AB - The volume fraction of red blood cells (RBCs) in a capillary affects the degree to which platelets are promoted to marginate to near a vessel wall and form blood clots. In this work we investigate the relationship between RBC hematocrit and platelet adhesion activity. We perform experiments flowing blood samples through a microfluidic channel coated with type 1 collagen and observe the rate at which platelets adhere to the wall. We compare these results with three-dimensional boundary integral simulations of a suspension of RBCs and platelets in a periodic channel where platelets can adhere to the wall. In both cases, we find that the rate of platelet adhesion varies greatly with the RBC hematocrit. We observe that the relative decrease in platelet activity as hematocrit falls shows a similar profile for simulation and experiment.

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The Effect of Hematocrit on Platelet Adhesion: Experiments and Simulations

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