Abstract
Method: We injected two drugs that modify the epigenome, the DNA methyltransferase inhibitor 5-aza-2́-deoxycytidine (5-aza-dC) and the histone deacetylase inhibitor trichostatin A (TSA), alone or in combination, into C57Bl/6 mice subjected to amputation through the mid-second phalanx of the third digit. Wound-site tissue was collected. Results: We observed increased staining of the stem cell markers Rex1 (Zfp42) and stem cell antigen-1 at digit amputation sites from drug-treated mice. Samples from 5-aza-dC plus TSA and TSA treated mice also showed increased proliferating cell nuclear antigen staining, a measure of cell proliferation. Drug treatments increased Msx1, but not Cyp26a1 or ALDH1a2 (RALDH2) mRNA. Conclusion: 5-aza-dC and TSA treatments stimulated cell proliferation at the amputation site, possibly via increased expression of genes involved in digit development and regeneration.
Original language | English |
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Pages (from-to) | 201-220 |
Number of pages | 20 |
Journal | Regenerative Medicine |
Volume | 5 |
Issue number | 2 |
DOIs | |
State | Published - Mar 2010 |
Externally published | Yes |
Keywords
- Cell proliferation
- Epigenetics
- Msx1
- Pharmacology
- Regeneration
- Regenerative medicine n retinoic acid n stem cells n transcription n wound healing