The phorbol ester TPA enhances A23187- but not carbachol-and high K+-induced catecholamine secretion from cultured bovine adrenal chromaffin cells

Kyoji Morita; Keith W. Brocklehurst; Stuart M. Tomares; Harvey B. Pollard

doi:10.1016/0006-291X(85)90181-0

The phorbol ester TPA enhances A23187- but not carbachol-and high K⁺-induced catecholamine secretion from cultured bovine adrenal chromaffin cells

Kyoji Morita^*, Keith W. Brocklehurst, Stuart M. Tomares, Harvey B. Pollard

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

The effect of the phorbol ester TPA on catecholamine secretion was studied in cultured bovine adrenal chromaffin cells. The pretreatment of chromaffin cells with TPA caused the enhancement of catecholamine secretion induced by the calcium ionophore, A23187. By contrast, neither carbachol-nor high K⁺-induced secretion was changed by TPA pretreatment. These results support the concept that protein kinase C plays an important role as a factor transducing the Ca²⁺ signal to the exocytotic process of catecholamine secretion in bovine adrenal chromaffin cells.

Original language	English
Pages (from-to)	511-516
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	129
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(85)90181-0
State	Published - 14 Jun 1985

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title = "The phorbol ester TPA enhances A23187- but not carbachol-and high K+-induced catecholamine secretion from cultured bovine adrenal chromaffin cells",

abstract = "The effect of the phorbol ester TPA on catecholamine secretion was studied in cultured bovine adrenal chromaffin cells. The pretreatment of chromaffin cells with TPA caused the enhancement of catecholamine secretion induced by the calcium ionophore, A23187. By contrast, neither carbachol-nor high K+-induced secretion was changed by TPA pretreatment. These results support the concept that protein kinase C plays an important role as a factor transducing the Ca2+ signal to the exocytotic process of catecholamine secretion in bovine adrenal chromaffin cells.",

author = "Kyoji Morita and Brocklehurst, \{Keith W.\} and Tomares, \{Stuart M.\} and Pollard, \{Harvey B.\}",

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The phorbol ester TPA enhances A23187- but not carbachol-and high K⁺-induced catecholamine secretion from cultured bovine adrenal chromaffin cells. / Morita, Kyoji; Brocklehurst, Keith W.; Tomares, Stuart M. et al.
In: Biochemical and Biophysical Research Communications, Vol. 129, No. 2, 14.06.1985, p. 511-516.

Research output: Contribution to journal › Article › peer-review

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AU - Morita, Kyoji

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AU - Pollard, Harvey B.

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The phorbol ester TPA enhances A23187- but not carbachol-and high K+-induced catecholamine secretion from cultured bovine adrenal chromaffin cells

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The phorbol ester TPA enhances A23187- but not carbachol-and high K⁺-induced catecholamine secretion from cultured bovine adrenal chromaffin cells