TY - JOUR
T1 - The PreQuine Platform
T2 - A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration
AU - Harper, Robert
AU - Ley, Benedikt
AU - Kabir, Md Alamgir
AU - Matulis, Graham
AU - von Seidlein, Lorenz
AU - Alam, Mohammad Shafiul
AU - Adhikari, Bipin
AU - Okech, Bernard A
AU - Williams, Alan L
AU - Price, Ric N
AU - von Fricken, Michael E
N1 - Publisher Copyright:
This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
PY - 2024/1
Y1 - 2024/1
N2 - Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.
AB - Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.
KW - Humans
KW - Glucosephosphate Dehydrogenase/metabolism
KW - Glucosephosphate Dehydrogenase Deficiency/diagnosis
KW - Hemoglobins/analysis
KW - Biosensing Techniques/methods
KW - Malaria, Vivax/diagnosis
KW - Aminoquinolines
UR - https://www.mendeley.com/catalogue/e30fa25f-b5e3-3427-836e-3ffd9155cf0d/
U2 - 10.1371/journal.pone.0297918
DO - 10.1371/journal.pone.0297918
M3 - Article
C2 - 38728310
SN - 1932-6203
VL - 19
SP - e0297918
JO - PLoS ONE
JF - PLoS ONE
IS - 5 May
M1 - e0297918
ER -