The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration

Robert Harper; Benedikt Ley; Md Alamgir Kabir; Graham Matulis; Lorenz von Seidlein; Mohammad Shafiul Alam; Bipin Adhikari; Bernard A Okech; Alan L Williams; Ric N Price; Michael E von Fricken

doi:10.1371/journal.pone.0297918

The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration

Robert Harper, Benedikt Ley, Md Alamgir Kabir, Graham Matulis, Lorenz von Seidlein, Mohammad Shafiul Alam, Bipin Adhikari, Bernard A Okech, Alan L Williams, Ric N Price, Michael E von Fricken

Research output: Contribution to journal › Article › peer-review

Abstract

Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.

Original language	American English
Article number	e0297918
Pages (from-to)	e0297918
Journal	PLoS ONE
Volume	19
Issue number	5 May
DOIs	https://doi.org/10.1371/journal.pone.0297918 https://doi.org/10.1371/journal.pone.0297918
State	Published - Jan 2024

Keywords

Humans
Glucosephosphate Dehydrogenase/metabolism
Glucosephosphate Dehydrogenase Deficiency/diagnosis
Hemoglobins/analysis
Biosensing Techniques/methods
Malaria, Vivax/diagnosis
Aminoquinolines

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Harper, R., Ley, B., Kabir, M. A., Matulis, G., von Seidlein, L., Alam, M. S., Adhikari, B., Okech, B. A., Williams, A. L., Price, R. N., & von Fricken, M. E. (2024). The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration. PLoS ONE, 19(5 May), e0297918. Article e0297918. https://doi.org/10.1371/journal.pone.0297918, https://doi.org/10.1371/journal.pone.0297918

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title = "The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration",

abstract = "Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.",

keywords = "Humans, Glucosephosphate Dehydrogenase/metabolism, Glucosephosphate Dehydrogenase Deficiency/diagnosis, Hemoglobins/analysis, Biosensing Techniques/methods, Malaria, Vivax/diagnosis, Aminoquinolines",

author = "Robert Harper and Benedikt Ley and Kabir, {Md Alamgir} and Graham Matulis and {von Seidlein}, Lorenz and Alam, {Mohammad Shafiul} and Bipin Adhikari and Okech, {Bernard A} and Williams, {Alan L} and Price, {Ric N} and {von Fricken}, {Michael E}",

note = "Publisher Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.",

year = "2024",

month = jan,

doi = "10.1371/journal.pone.0297918",

language = "American English",

volume = "19",

pages = "e0297918",

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Harper, R, Ley, B, Kabir, MA, Matulis, G, von Seidlein, L, Alam, MS, Adhikari, B, Okech, BA , Williams, AL, Price, RN & von Fricken, ME 2024, 'The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration', PLoS ONE, vol. 19, no. 5 May, e0297918, pp. e0297918. https://doi.org/10.1371/journal.pone.0297918, https://doi.org/10.1371/journal.pone.0297918

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AU - Harper, Robert

AU - Ley, Benedikt

AU - Kabir, Md Alamgir

AU - Matulis, Graham

AU - von Seidlein, Lorenz

AU - Alam, Mohammad Shafiul

AU - Adhikari, Bipin

AU - Okech, Bernard A

AU - Williams, Alan L

AU - Price, Ric N

AU - von Fricken, Michael E

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PY - 2024/1

Y1 - 2024/1

N2 - Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.

AB - Quantitative diagnosis of glucose-6-phosphate dehydrogenase (G6PD) deficiency is essential for the safe administration of 8-aminoquinoline based radical cure for the treatment of Plasmodium vivax infections. Here, we present the PreQuine Platform (IVDS, USA), a quantitative biosensor that uses a dual-analyte assay for the simultaneous measurement of Hemoglobin (Hgb) levels and G6PD enzyme activity within the same sample. The platform relies on a downloadable mobile application. The device requires 10μl of whole blood and works with a reflectance-based meter. Comparing the G6PD measurement normalized by Hgb of 12 samples from the PreQuine Platform with reference measurements methods (spectrophotometry, Pointe Scientific, USA and hemoglobin meter, HemoCue, Sweden) showed a positive and significant agreement with a slope of 1.0091 and an intercept of -0.0379 under laboratory conditions. Next steps will be to conduct field trials in Bangladesh, Cambodia, and the USA to assess diagnostic performance, user friendliness and acceptance.

KW - Humans

KW - Glucosephosphate Dehydrogenase/metabolism

KW - Glucosephosphate Dehydrogenase Deficiency/diagnosis

KW - Hemoglobins/analysis

KW - Biosensing Techniques/methods

KW - Malaria, Vivax/diagnosis

KW - Aminoquinolines

UR - https://www.mendeley.com/catalogue/e30fa25f-b5e3-3427-836e-3ffd9155cf0d/

U2 - 10.1371/journal.pone.0297918

DO - 10.1371/journal.pone.0297918

M3 - Article

C2 - 38728310

SN - 1932-6203

VL - 19

SP - e0297918

JO - PLoS ONE

JF - PLoS ONE

IS - 5 May

M1 - e0297918

ER -

The PreQuine Platform: A novel diagnostic tool for measuring glucose-6-phosphate dehydrogenase (G6PD) activity and hemoglobin concentration

Abstract

Keywords

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