The presence of F3-F2a1 dimers and F1 oligomers in chromatin

William M. Bonner; Harvey B. Pollard

doi:10.1016/0006-291X(75)90250-8

The presence of F3-F2a1 dimers and F1 oligomers in chromatin

William M. Bonner^*
, Harvey B. Pollard

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

The oligomeric structure of histones in nuclei and chromatin has been studied by crosslinking nuclei and chromatin with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. Crosslinked histones were detected as new high molecular weight components on SDS gels, and the protomers of the crosslinked histones were identified by their characteristic ¹²⁵I-fingerprints. The results show that a considerable portion of histones F3 and F2a1 exist in nuclei and chromatin as an F3-F2a1 dimer. Evidence is presented that histone F1 probably exists in chromatin as large oligomers.

Original language	English
Pages (from-to)	282-288
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	64
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(75)90250-8
State	Published - 5 May 1975

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title = "The presence of F3-F2a1 dimers and F1 oligomers in chromatin",

abstract = "The oligomeric structure of histones in nuclei and chromatin has been studied by crosslinking nuclei and chromatin with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. Crosslinked histones were detected as new high molecular weight components on SDS gels, and the protomers of the crosslinked histones were identified by their characteristic 125I-fingerprints. The results show that a considerable portion of histones F3 and F2a1 exist in nuclei and chromatin as an F3-F2a1 dimer. Evidence is presented that histone F1 probably exists in chromatin as large oligomers.",

author = "Bonner, \{William M.\} and Pollard, \{Harvey B.\}",

year = "1975",

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T1 - The presence of F3-F2a1 dimers and F1 oligomers in chromatin

AU - Bonner, William M.

AU - Pollard, Harvey B.

PY - 1975/5/5

Y1 - 1975/5/5

N2 - The oligomeric structure of histones in nuclei and chromatin has been studied by crosslinking nuclei and chromatin with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. Crosslinked histones were detected as new high molecular weight components on SDS gels, and the protomers of the crosslinked histones were identified by their characteristic 125I-fingerprints. The results show that a considerable portion of histones F3 and F2a1 exist in nuclei and chromatin as an F3-F2a1 dimer. Evidence is presented that histone F1 probably exists in chromatin as large oligomers.

AB - The oligomeric structure of histones in nuclei and chromatin has been studied by crosslinking nuclei and chromatin with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. Crosslinked histones were detected as new high molecular weight components on SDS gels, and the protomers of the crosslinked histones were identified by their characteristic 125I-fingerprints. The results show that a considerable portion of histones F3 and F2a1 exist in nuclei and chromatin as an F3-F2a1 dimer. Evidence is presented that histone F1 probably exists in chromatin as large oligomers.

UR - https://www.scopus.com/pages/publications/0016843483

U2 - 10.1016/0006-291X(75)90250-8

DO - 10.1016/0006-291X(75)90250-8

M3 - Article

C2 - 1147923

AN - SCOPUS:0016843483

SN - 0006-291X

VL - 64

SP - 282

EP - 288

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

The presence of F3-F2a1 dimers and F1 oligomers in chromatin

Abstract

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