The three-chamber choice behavioral task using zebrafish as a model system

Cassie J. Rowe*, Mikayla Crowley-Perry, Elizabeth McCarthy, Terry L. Davidson, Victoria P. Connaughton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Neurodegenerative diseases are age-dependent, debilitating, and incurable. Recent reports have also correlated hyperglycemia with changes in memory and/or cognitive impairment. We have modified and developed a three-chamber choice cognitive task similar to that used with rodents for use with hyperglycemic zebrafish. The testing chamber consists of a centrally located starting chamber and two choice compartments on either side, with a shoal of conspecifics used as the reward. We provide data showing that once acquired, zebrafish remember the task at least 8 weeks later. Our data indicate that zebrafish respond robustly to this reward, and we have identified cognitive deficits in hyperglycemic fish after 4 weeks of treatment. This behavioral assay may also be applicable to other studies related to cognition and memory.

Original languageEnglish
Article numbere61934
JournalJournal of Visualized Experiments
Issue number170
StatePublished - 2021
Externally publishedYes


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