TY - JOUR
T1 - The use of Nanotrap particles for biodefense and emerging infectious disease diagnostics
AU - Shafagati, Nazly
AU - Patanarut, Alexis
AU - Luchini, Alessandra
AU - Lundberg, Lindsay
AU - Bailey, Charles
AU - Petricoin, Emanuel
AU - Liotta, Lance
AU - Narayanan, Aarthi
AU - Lepene, Benjamin
AU - Kehn-Hall, Kylene
PY - 2014/7
Y1 - 2014/7
N2 - Detection of early infectious disease may be challenging due to the low copy number of organisms present. To overcome this limitation and rapidly measure low concentrations of the pathogen, we developed a novel technology: Nanotrap particles, which are designed to capture, concentrate, and protect biomarkers from complex biofluids. Nanotrap particles are thermoresponsive hydrogels that are capable of antigen capture through the coupling of affinity baits to the particles. Here, we describe recent findings demonstrating that Nanotrap particles are able to capture live infectious virus, viral RNA, and viral proteins. Capture is possible even in complex mixtures such as serum and allows the concentration and protection of these analytes, providing increased performance of downstream assays. The Nanotrap particles are a versatile sample preparation technology that has far reaching implications for biomarker discovery and diagnostic assays.
AB - Detection of early infectious disease may be challenging due to the low copy number of organisms present. To overcome this limitation and rapidly measure low concentrations of the pathogen, we developed a novel technology: Nanotrap particles, which are designed to capture, concentrate, and protect biomarkers from complex biofluids. Nanotrap particles are thermoresponsive hydrogels that are capable of antigen capture through the coupling of affinity baits to the particles. Here, we describe recent findings demonstrating that Nanotrap particles are able to capture live infectious virus, viral RNA, and viral proteins. Capture is possible even in complex mixtures such as serum and allows the concentration and protection of these analytes, providing increased performance of downstream assays. The Nanotrap particles are a versatile sample preparation technology that has far reaching implications for biomarker discovery and diagnostic assays.
KW - Diagnostics
KW - Influenza
KW - Rift valley fever virus
KW - Venezuelan equine encephalitis virus
UR - http://www.scopus.com/inward/record.url?scp=84904250910&partnerID=8YFLogxK
U2 - 10.1111/2049-632X.12136
DO - 10.1111/2049-632X.12136
M3 - Review article
C2 - 24449537
AN - SCOPUS:84904250910
SN - 2049-632X
VL - 71
SP - 164
EP - 176
JO - Pathogens and Disease
JF - Pathogens and Disease
IS - 2
ER -