TY - JOUR
T1 - Tissue Source and Cell Expansion Condition Influence Phenotypic Changes of Adipose-Derived Stem Cells
AU - Mangum, Lauren H.
AU - Natesan, Shanmugasundaram
AU - Stone, Randolph
AU - Wrice, Nicole L.
AU - Larson, David A.
AU - Florell, Kyle F.
AU - Christy, Barbara A.
AU - Herzig, Maryanne C.
AU - Cap, Andrew P.
AU - Christy, Robert J.
N1 - Publisher Copyright:
© 2017 Lauren H. Mangum et al.
PY - 2017
Y1 - 2017
N2 - Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH). Most (95-99%) cells cultured in FBS were stained positive for CD73, CD90, CD105, and CD142. FBS supplementation was associated with increased triglyceride content and expression of adipogenic genes. Culture in hPL significantly decreased surface staining of CD105 by 31% and 48% and CD142 by 27% and 35% in HAP and BH, respectively (p<0.05). Culture of BH-ASCs in hPL also increased expression of markers of osteogenesis and increased ALP activity. These data indicate that application of ASCs for wound healing may be influenced by ASC source as well as culture conditions used to expand them. As such, these factors must be taken into consideration before ASCs are used for regenerative purposes.
AB - Stem cells derived from the subcutaneous adipose tissue of debrided burned skin represent an appealing source of adipose-derived stem cells (ASCs) for regenerative medicine. Traditional tissue culture uses fetal bovine serum (FBS), which complicates utilization of ASCs in human medicine. Human platelet lysate (hPL) is one potential xeno-free, alternative supplement for use in ASC culture. In this study, adipogenic and osteogenic differentiation in media supplemented with 10% FBS or 10% hPL was compared in human ASCs derived from abdominoplasty (HAP) or from adipose associated with debrided burned skin (BH). Most (95-99%) cells cultured in FBS were stained positive for CD73, CD90, CD105, and CD142. FBS supplementation was associated with increased triglyceride content and expression of adipogenic genes. Culture in hPL significantly decreased surface staining of CD105 by 31% and 48% and CD142 by 27% and 35% in HAP and BH, respectively (p<0.05). Culture of BH-ASCs in hPL also increased expression of markers of osteogenesis and increased ALP activity. These data indicate that application of ASCs for wound healing may be influenced by ASC source as well as culture conditions used to expand them. As such, these factors must be taken into consideration before ASCs are used for regenerative purposes.
UR - http://www.scopus.com/inward/record.url?scp=85029217671&partnerID=8YFLogxK
U2 - 10.1155/2017/7108458
DO - 10.1155/2017/7108458
M3 - Article
AN - SCOPUS:85029217671
SN - 1687-9678
VL - 2017
JO - Stem Cells International
JF - Stem Cells International
M1 - 7108458
ER -