TY - JOUR
T1 - Tissue-Specific Effects of Esophageal Extracellular Matrix
AU - Keane, Timothy J.
AU - DeWard, Aaron
AU - Londono, Ricardo
AU - Saldin, Lindsey T.
AU - Castleton, Arthur A.
AU - Carey, Lisa
AU - Nieponice, Alejandro
AU - Lagasse, Eric
AU - Badylak, Stephen F.
N1 - Publisher Copyright:
Copyright 2015, Mary Ann Liebert, Inc.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - Biologic scaffolds composed of extracellular matrix (ECM) have been used to facilitate repair or remodeling of numerous tissues, including the esophagus. The theoretically ideal scaffold for tissue repair is the ECM derived from the particular tissue to be treated, that is, site-specific or homologous ECM. The preference or potential advantage for the use of site-specific ECM remains unknown in the esophageal location. The objective of the present study was to characterize the in vitro cellular response and in vivo host response to a homologous esophageal ECM (eECM) versus nonhomologous ECMs derived from small intestinal submucosa and urinary bladder. The in vitro response of esophageal stem cells was characterized by migration, proliferation, and three-dimensional (3D) organoid formation assays. The in vivo remodeling response was evaluated in a rat model of esophageal mucosal resection. Results of the study showed that the eECM retains favorable tissue-specific characteristics that enhance the migration of esophageal stem cells and supports the formation of 3D organoids to a greater extent than heterologous ECMs. Implantation of eECM facilitates the remodeling of esophageal mucosa following mucosal resection, but no distinct advantage versus heterologous ECM could be identified.
AB - Biologic scaffolds composed of extracellular matrix (ECM) have been used to facilitate repair or remodeling of numerous tissues, including the esophagus. The theoretically ideal scaffold for tissue repair is the ECM derived from the particular tissue to be treated, that is, site-specific or homologous ECM. The preference or potential advantage for the use of site-specific ECM remains unknown in the esophageal location. The objective of the present study was to characterize the in vitro cellular response and in vivo host response to a homologous esophageal ECM (eECM) versus nonhomologous ECMs derived from small intestinal submucosa and urinary bladder. The in vitro response of esophageal stem cells was characterized by migration, proliferation, and three-dimensional (3D) organoid formation assays. The in vivo remodeling response was evaluated in a rat model of esophageal mucosal resection. Results of the study showed that the eECM retains favorable tissue-specific characteristics that enhance the migration of esophageal stem cells and supports the formation of 3D organoids to a greater extent than heterologous ECMs. Implantation of eECM facilitates the remodeling of esophageal mucosa following mucosal resection, but no distinct advantage versus heterologous ECM could be identified.
UR - http://www.scopus.com/inward/record.url?scp=84940702396&partnerID=8YFLogxK
U2 - 10.1089/ten.tea.2015.0322
DO - 10.1089/ten.tea.2015.0322
M3 - Article
C2 - 26192009
AN - SCOPUS:84940702396
SN - 1937-3341
VL - 21
SP - 2293
EP - 2300
JO - Tissue Engineering - Part A.
JF - Tissue Engineering - Part A.
IS - 17-18
ER -