TNFα-308G/A polymorphism as a risk factor for HPV associated Cervical Cancer in Indian population

Indu Kohaar, Nisha Thakur, Sudha Salhan, Swaraj Batra, Veena Singh, Anita Sharma, Pushpa Sodhani, B. C. Das, Debi P. Sarkar, Mausumi Bharadwaj*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

67 Scopus citations


Background: Investigation of the potential association of single nucleotide polymorphisms (SNPs) at -308 G/A and -238 G/A of Tumor necrosis factor α (TNFα) with susceptibility to HPV-16 associated cervical cancer in Indian women. Methods: The study included 165 histologically confirmed cases with 45 precancer and 120 cancer patients and an equal number (165) of healthy controls with normal cervical cytology. PCR-RFLP was employed to analyze TNFα promoter polymorphisms, which were confirmed by direct sequencing. Both patients and controls were screened for Human Papillomavirus (HPV) infection. Results: The frequency of -308 A allele in TNFα was significantly higher in cases compared with control subjects (21% in cases vs. 9% in controls; p<0.01), with an odds ratio of 2.7 (95% CI = 1.41-5.15). Also, women carrying A allele for this locus presented 3 times increased susceptibility to HPV 16 infection as evident from carrier genotype distribution between HPV positive cases and control subjects (24% in HPV positive cases vs. 9% in controls; p<0.01; OR = 3.1; 95% CI = 1.60-6.03). No such association was found for TNFα-238 (G/A) polymorphism with the risk of development of cervical cancer. Conclusion: It suggests that SNP at -308 (G/A) of TNFα promoter may represent an increased risk for HPV infection and development of cervical cancer in Indian women.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalCellular Oncology
Issue number3
StatePublished - 2007
Externally publishedYes


  • -238 promoter polymorphism
  • -308 promoter polymorphism
  • Cervical cancer
  • Cervical precancer
  • Genetic susceptibility
  • Human papillomavirus
  • Single nucleotide polymorphism
  • Tumor necrosis factor α (TNFα)


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