Two forms of the GABAA receptor distinguished by anion-exchange chromatography

John R. Moffett; Aryan Namboodiri; Joseph H. Neale

doi:10.1016/0014-5793(89)81245-1

Two forms of the GABA_A receptor distinguished by anion-exchange chromatography

John R. Moffett^*
, Aryan Namboodiri
, Joseph H. Neale

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The GABA_A receptor complex was solubilized from rat brain membranes in Triton X-100, enriched by 1012-S affinity chromatography, and subjected to DEAE anion-exchange chromatography. Two forms were distinguished by their differential elution during this HPLC with a KCl gradient. They displayed similar [³H]muscimol- and [³H]flunitrazepam-binding characteristics, as well as [³H]flunitrazepam-binding inhibition by CL 218872. Rechromatography of these distinct ionic forms indicated that they were not in dynamic equilibrium during chromatography. Resolution of these two pharmacologically similar populations of GABA_A receptor by anion-exchange HPLC suggests that they differ in charge densities, a condition which may reflect differing glycosylation or phosporylation states of the complex.

Original language	English
Pages (from-to)	81-85
Number of pages	5
Journal	FEBS Letters
Volume	247
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(89)81245-1
State	Published - 10 Apr 1989

Keywords

Aminobutyric acid receptor, γ-
Anion-exchange HPLC
Benzodiazepine
CL 218872
Receptor heterogeneity

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10.1016/0014-5793(89)81245-1

Cite this

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title = "Two forms of the GABAA receptor distinguished by anion-exchange chromatography",

abstract = "The GABAA receptor complex was solubilized from rat brain membranes in Triton X-100, enriched by 1012-S affinity chromatography, and subjected to DEAE anion-exchange chromatography. Two forms were distinguished by their differential elution during this HPLC with a KCl gradient. They displayed similar [3H]muscimol- and [3H]flunitrazepam-binding characteristics, as well as [3H]flunitrazepam-binding inhibition by CL 218872. Rechromatography of these distinct ionic forms indicated that they were not in dynamic equilibrium during chromatography. Resolution of these two pharmacologically similar populations of GABAA receptor by anion-exchange HPLC suggests that they differ in charge densities, a condition which may reflect differing glycosylation or phosporylation states of the complex.",

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AB - The GABAA receptor complex was solubilized from rat brain membranes in Triton X-100, enriched by 1012-S affinity chromatography, and subjected to DEAE anion-exchange chromatography. Two forms were distinguished by their differential elution during this HPLC with a KCl gradient. They displayed similar [3H]muscimol- and [3H]flunitrazepam-binding characteristics, as well as [3H]flunitrazepam-binding inhibition by CL 218872. Rechromatography of these distinct ionic forms indicated that they were not in dynamic equilibrium during chromatography. Resolution of these two pharmacologically similar populations of GABAA receptor by anion-exchange HPLC suggests that they differ in charge densities, a condition which may reflect differing glycosylation or phosporylation states of the complex.

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