TY - JOUR
T1 - Two unique recombinant forms identified in incident HIV type 1 infections in Thai blood donors
AU - Rutvisuttinunt, Wiriya
AU - Sirivichayakul, Sunee
AU - Oota, Sineenart
AU - Assawadarachai, Vatcharain
AU - Poltavee, Kultida
AU - Savadsuk, Hathairat
AU - Pattanachaiwit, Supanit
AU - Chaemchuen, Suwittra
AU - Arroyo, Miguel A.
AU - Paris, Robert M.
AU - Michael, Nelson L.
AU - Kim, Jerome H.
AU - Ruxrungtham, Kiat
AU - De Souza, Mark
AU - Phanuphak, Praphan
AU - Tovanabutra, Sodsai
PY - 2012/12/1
Y1 - 2012/12/1
N2 - HIV-1 genetic diversity of recently seroconverting (<12 months) Thai repeated blood donors attending the National Blood Centre, Thai Red Cross Society (NBC, TRCS) from September 2007 until March 2008 was assessed. Ten HIV-1 recent seroconvertors (10/239,134 donations) were identified during the study period. The estimated median time to seroconversion was 67.3 days (range: 45.5-102.0 days), and viral load ranged from 307 to 341,805 copies HIV-1 RNA/ml. MHAbce, a real-time-based PCR genotyping assay, identified six CRF01-AE, two CRF01-AE/B recombinants, one subtype B, and one CRF01-AE/B dual infection. Nine samples were further characterized by full genome sequencing, identifying CRF01-AE (N=6), unique CRF01-AE/B recombinants (N=2), and subtype B (N=1). One recombinant contained 13 breakpoints located in gag, pol, vif, vpr, env, and nef while the other recombinant contained 10 breakpoints located in pol, vif, env, and nef. This study found two unique CRF01B recombinants circulating in 10 recent HIV-1-positive subjects from a blood donor population in Thailand.
AB - HIV-1 genetic diversity of recently seroconverting (<12 months) Thai repeated blood donors attending the National Blood Centre, Thai Red Cross Society (NBC, TRCS) from September 2007 until March 2008 was assessed. Ten HIV-1 recent seroconvertors (10/239,134 donations) were identified during the study period. The estimated median time to seroconversion was 67.3 days (range: 45.5-102.0 days), and viral load ranged from 307 to 341,805 copies HIV-1 RNA/ml. MHAbce, a real-time-based PCR genotyping assay, identified six CRF01-AE, two CRF01-AE/B recombinants, one subtype B, and one CRF01-AE/B dual infection. Nine samples were further characterized by full genome sequencing, identifying CRF01-AE (N=6), unique CRF01-AE/B recombinants (N=2), and subtype B (N=1). One recombinant contained 13 breakpoints located in gag, pol, vif, vpr, env, and nef while the other recombinant contained 10 breakpoints located in pol, vif, env, and nef. This study found two unique CRF01B recombinants circulating in 10 recent HIV-1-positive subjects from a blood donor population in Thailand.
UR - http://www.scopus.com/inward/record.url?scp=84870000174&partnerID=8YFLogxK
U2 - 10.1089/aid.2011.0339
DO - 10.1089/aid.2011.0339
M3 - Article
C2 - 22587412
AN - SCOPUS:84870000174
SN - 0889-2229
VL - 28
SP - 1703
EP - 1711
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 12
ER -