Two unique recombinant forms identified in incident HIV type 1 infections in Thai blood donors

Wiriya Rutvisuttinunt*, Sunee Sirivichayakul, Sineenart Oota, Vatcharain Assawadarachai, Kultida Poltavee, Hathairat Savadsuk, Supanit Pattanachaiwit, Suwittra Chaemchuen, Miguel A. Arroyo, Robert M. Paris, Nelson L. Michael, Jerome H. Kim, Kiat Ruxrungtham, Mark De Souza, Praphan Phanuphak, Sodsai Tovanabutra

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


HIV-1 genetic diversity of recently seroconverting (<12 months) Thai repeated blood donors attending the National Blood Centre, Thai Red Cross Society (NBC, TRCS) from September 2007 until March 2008 was assessed. Ten HIV-1 recent seroconvertors (10/239,134 donations) were identified during the study period. The estimated median time to seroconversion was 67.3 days (range: 45.5-102.0 days), and viral load ranged from 307 to 341,805 copies HIV-1 RNA/ml. MHAbce, a real-time-based PCR genotyping assay, identified six CRF01-AE, two CRF01-AE/B recombinants, one subtype B, and one CRF01-AE/B dual infection. Nine samples were further characterized by full genome sequencing, identifying CRF01-AE (N=6), unique CRF01-AE/B recombinants (N=2), and subtype B (N=1). One recombinant contained 13 breakpoints located in gag, pol, vif, vpr, env, and nef while the other recombinant contained 10 breakpoints located in pol, vif, env, and nef. This study found two unique CRF01B recombinants circulating in 10 recent HIV-1-positive subjects from a blood donor population in Thailand.

Original languageEnglish
Pages (from-to)1703-1711
Number of pages9
JournalAIDS Research and Human Retroviruses
Issue number12
StatePublished - 1 Dec 2012
Externally publishedYes


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