Upregulation of HLA class-I gene transcription in K562 cells by analogs of splenopentin (SP-5)

Moitreyee Chatterjee-Kishore; Suraksha Agrawal; V. K. Singh; K. B. Mathur; Shyam S. Agarwal

doi:10.1080/15216549700201541

Upregulation of HLA class-I gene transcription in K562 cells by analogs of splenopentin (SP-5)

Moitreyee Chatterjee-Kishore, Suraksha Agrawal, V. K. Singh, K. B. Mathur, Shyam S. Agarwal^*

^*Corresponding author for this work

Pharmacology and Molecular Therapeutics

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

We report here the capacity of three analogs of Splenopentin, to up regulate transcription of the HLA-B7 gene in the K562 cell line. These cells normally do not transcribe the HLA class I genes. The Splenopentin analogs used in this study are effective at very low molar concentrations and are non-toxic to cells at these levels. Electrophoretic Mobility Shift assays indicate that this transcriptional up regulation of HLA class I genes may be related to the appearance of novel class I promoter binding factors induced in the nuclei of treated cells.

Original language	English
Pages (from-to)	521-528
Number of pages	8
Journal	Biochemistry and Molecular Biology International
Volume	41
Issue number	3
DOIs	https://doi.org/10.1080/15216549700201541
State	Published - Mar 1997

Keywords

HLA class I
NF-κB
Splenopentin
Transcription

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10.1080/15216549700201541

Cite this

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abstract = "We report here the capacity of three analogs of Splenopentin, to up regulate transcription of the HLA-B7 gene in the K562 cell line. These cells normally do not transcribe the HLA class I genes. The Splenopentin analogs used in this study are effective at very low molar concentrations and are non-toxic to cells at these levels. Electrophoretic Mobility Shift assays indicate that this transcriptional up regulation of HLA class I genes may be related to the appearance of novel class I promoter binding factors induced in the nuclei of treated cells.",

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AU - Mathur, K. B.

AU - Agarwal, Shyam S.

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AB - We report here the capacity of three analogs of Splenopentin, to up regulate transcription of the HLA-B7 gene in the K562 cell line. These cells normally do not transcribe the HLA class I genes. The Splenopentin analogs used in this study are effective at very low molar concentrations and are non-toxic to cells at these levels. Electrophoretic Mobility Shift assays indicate that this transcriptional up regulation of HLA class I genes may be related to the appearance of novel class I promoter binding factors induced in the nuclei of treated cells.

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KW - NF-κB

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KW - Transcription

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