Uridine labeling of human lymphocytes: Differential uptake by T and B cells

David W. Scott; Shelby H. Josephs

doi:10.1016/0008-8749(75)90084-2

Uridine labeling of human lymphocytes: Differential uptake by T and B cells

David W. Scott^*, Shelby H. Josephs

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Human peripheral blood lymphocytes were labeled with [³H]uridine and separated into immunoglobulin (Ig)-positive-enriched and Ig-negative populations by rosetting and Ficoll sedimentation. The Ig-negative (T cell-rich) fraction was found to be more heavily labeled than the B cell-enriched population, in agreement with previous results in rats. Combined autoradiography and rosetting confirmed the differential uridine labeling of T and B cells. Incorporation of cytidine and adenosine by T and B cell-enriched populations showed similar but less dramatic differential labeling.

Original language	English
Pages (from-to)	64-68
Number of pages	5
Journal	Cellular Immunology
Volume	20
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(75)90084-2
State	Published - Nov 1975
Externally published	Yes

Access to Document

10.1016/0008-8749(75)90084-2

Cite this

@article{1f7029a721ff46f2ab6e1c0f20970094,

title = "Uridine labeling of human lymphocytes: Differential uptake by T and B cells",

abstract = "Human peripheral blood lymphocytes were labeled with [3H]uridine and separated into immunoglobulin (Ig)-positive-enriched and Ig-negative populations by rosetting and Ficoll sedimentation. The Ig-negative (T cell-rich) fraction was found to be more heavily labeled than the B cell-enriched population, in agreement with previous results in rats. Combined autoradiography and rosetting confirmed the differential uridine labeling of T and B cells. Incorporation of cytidine and adenosine by T and B cell-enriched populations showed similar but less dramatic differential labeling.",

author = "Scott, {David W.} and Josephs, {Shelby H.}",

note = "Funding Information: 1 Supported by USPHS Grant No. AI-10716. 2 Abbreviations used in this paper : E, sheep erythrocytes ; FCS, fetal calf serum ; Ig, immunoglobulin ; PBL, peripheral blood lymphocytes ; RAHGG, rabbit anti-human y-globulin.",

year = "1975",

month = nov,

doi = "10.1016/0008-8749(75)90084-2",

language = "English",

volume = "20",

pages = "64--68",

journal = "Cellular Immunology",

issn = "0008-8749",

number = "1",

}

TY - JOUR

T1 - Uridine labeling of human lymphocytes

T2 - Differential uptake by T and B cells

AU - Scott, David W.

AU - Josephs, Shelby H.

N1 - Funding Information: 1 Supported by USPHS Grant No. AI-10716. 2 Abbreviations used in this paper : E, sheep erythrocytes ; FCS, fetal calf serum ; Ig, immunoglobulin ; PBL, peripheral blood lymphocytes ; RAHGG, rabbit anti-human y-globulin.

PY - 1975/11

Y1 - 1975/11

N2 - Human peripheral blood lymphocytes were labeled with [3H]uridine and separated into immunoglobulin (Ig)-positive-enriched and Ig-negative populations by rosetting and Ficoll sedimentation. The Ig-negative (T cell-rich) fraction was found to be more heavily labeled than the B cell-enriched population, in agreement with previous results in rats. Combined autoradiography and rosetting confirmed the differential uridine labeling of T and B cells. Incorporation of cytidine and adenosine by T and B cell-enriched populations showed similar but less dramatic differential labeling.

AB - Human peripheral blood lymphocytes were labeled with [3H]uridine and separated into immunoglobulin (Ig)-positive-enriched and Ig-negative populations by rosetting and Ficoll sedimentation. The Ig-negative (T cell-rich) fraction was found to be more heavily labeled than the B cell-enriched population, in agreement with previous results in rats. Combined autoradiography and rosetting confirmed the differential uridine labeling of T and B cells. Incorporation of cytidine and adenosine by T and B cell-enriched populations showed similar but less dramatic differential labeling.

UR - http://www.scopus.com/inward/record.url?scp=0016800977&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(75)90084-2

DO - 10.1016/0008-8749(75)90084-2

M3 - Article

C2 - 1081910

AN - SCOPUS:0016800977

SN - 0008-8749

VL - 20

SP - 64

EP - 68

JO - Cellular Immunology

JF - Cellular Immunology

IS - 1

ER -

Uridine labeling of human lymphocytes: Differential uptake by T and B cells

Abstract

Access to Document

Fingerprint

Cite this