Urine analysis and protein networking identify met as a marker of metastatic prostate cancer

Andrea L. Russo, Kimberly Jedlicka, Meredith Wernick, Debbie McNally, Melissa Kirk, Mary Sproull, Sharon Smith, Uma Shankavaram, Aradhana Kaushal, William D. Figg, William Dahut, Deborah Citrin, Donald P. Bottaro, Paul S. Albert, Philip J. Tofilon, Kevin Camphausen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Purpose: Metastatic prostate cancer is a major cause of death of men in the United States. Expression of met, a receptor tyrosine kinase, has been associatedwithprogressionof prostate cancer. Experimental Design: To investigate met as a biomarker of disease progression, urinary met was evaluated via ELISA inmen with localized (n = 75) and metastatic (n = 81) prostate cancer. Boxplot analysis was used to compare the distribution of met values between each group.We estimated a receiver operating characteristic curve and the associated area under the curve to summarize the diagnostic accuracy of met for distinguishing between localized and metastatic disease. Protein-protein interaction networking via yeast two-hybrid technology supplemented by Ingenuity Pathway Analysis and Human Interactome was used to elucidate proteins and pathways related to met that may contribute to progression of disease. Results: Met distributionwas significantly different between the metastatic group and the group with localized prostate cancer and people with no evidence of cancer (P < 0.0001).The area under the curve for localized and metastatic disease was 0.90, with a 95% confidence interval of 0.84 to 0.95.Yeast two-hybrid technology, Ingenuity PathwayAnalysis, and Human Interactome identified 89 proteins that interact with met, of which 40 have previously been associated with metastatic prostate cancer. Conclusion: Urinary met may provide a noninvasive biomarker indicative of metastatic prostate cancerandmay be a central regulator ofmultiple pathways involved in prostate cancer progression.

Original languageEnglish
Pages (from-to)4292-4298
Number of pages7
JournalClinical Cancer Research
Issue number13
StatePublished - 1 Jul 2009
Externally publishedYes


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