TY - JOUR
T1 - Urine cytokines suggest an inflammatory response in the overactive bladder
T2 - A pilot study
AU - Tyagi, Pradeep
AU - Barclay, Derek
AU - Zamora, Ruben
AU - Yoshimura, Naoki
AU - Peters, Kenneth
AU - Vodovotz, Yoram
AU - Chancellor, Michael
N1 - Funding Information:
Acknowledgments We would like to thank Drs. Bruce Jacob and Wendy Leng for helping with urine collection. This study was supported in part by a research grant from Pfizer and National Institute on Disability Rehabilitation Research grant H133E070024.
PY - 2010/9
Y1 - 2010/9
N2 - Purpose: To study the hypothesis of detecting bladder inflammation associated with overactive bladder (OAB) through altered urine levels of cytokines, chemokines, and growth factors. Methods: Midstream urine specimens were collected from a prospective study done on eight asymptomatic control subjects and 17 idiopathic OAB patients. The urine was analyzed by a multiplex panel screen for 12 chemokines, cytokines, growth factors, and soluble receptors using Luminex™ xMAP® technology. Protein concentration values were normalized to the levels of creatinine. Results: This analysis revealed a significant elevation of seven key proteins in the urine of OAB patients relative to controls (P < 0.05). A greater than tenfold elevation was measured in OAB, relative to controls, in the levels of monocyte chemotactic protein-1 (MCP-1), soluble fraction of the CD40 ligand (sCD40L) in urine was obtained from OAB patients relative to controls. At least five fold elevations were detected in the levels of macrophage inflammatory protein (MIP-1β), IL-12p70/p40, IL-5, epidermal growth factor (EGF), and growth-related oncogene GRO-α compared to controls. Significant threefold elevation was also noticed in the urine levels of sIL-2Rα, and IL-10 in the OAB group. The levels of the remaining proteins tested were not statistically significantly different from control values. Conclusions: The presence of elevated levels in urine of inflammatory biomarkers involved in inflammation and tissue repair suggests a role for inflammation in OAB, and may help in diagnosis and treatment of this disease.
AB - Purpose: To study the hypothesis of detecting bladder inflammation associated with overactive bladder (OAB) through altered urine levels of cytokines, chemokines, and growth factors. Methods: Midstream urine specimens were collected from a prospective study done on eight asymptomatic control subjects and 17 idiopathic OAB patients. The urine was analyzed by a multiplex panel screen for 12 chemokines, cytokines, growth factors, and soluble receptors using Luminex™ xMAP® technology. Protein concentration values were normalized to the levels of creatinine. Results: This analysis revealed a significant elevation of seven key proteins in the urine of OAB patients relative to controls (P < 0.05). A greater than tenfold elevation was measured in OAB, relative to controls, in the levels of monocyte chemotactic protein-1 (MCP-1), soluble fraction of the CD40 ligand (sCD40L) in urine was obtained from OAB patients relative to controls. At least five fold elevations were detected in the levels of macrophage inflammatory protein (MIP-1β), IL-12p70/p40, IL-5, epidermal growth factor (EGF), and growth-related oncogene GRO-α compared to controls. Significant threefold elevation was also noticed in the urine levels of sIL-2Rα, and IL-10 in the OAB group. The levels of the remaining proteins tested were not statistically significantly different from control values. Conclusions: The presence of elevated levels in urine of inflammatory biomarkers involved in inflammation and tissue repair suggests a role for inflammation in OAB, and may help in diagnosis and treatment of this disease.
KW - Biomarker
KW - Cytokines
KW - Overactive bladder
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=77956547107&partnerID=8YFLogxK
U2 - 10.1007/s11255-009-9647-5
DO - 10.1007/s11255-009-9647-5
M3 - Article
C2 - 19784793
AN - SCOPUS:77956547107
SN - 0301-1623
VL - 42
SP - 629
EP - 635
JO - International Urology and Nephrology
JF - International Urology and Nephrology
IS - 3
ER -