Use of charcoal adsorption for the microassay of Acetyl-CoA hydrolase

M. A.A. Namboodiri; D. C. Klein

doi:10.1016/S0003-2697(79)80145-1

Use of charcoal adsorption for the microassay of Acetyl-CoA hydrolase

M. A.A. Namboodiri^*
, D. C. Klein

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

A sensitive and rapid radiochemical micromethod is described for measuring the activity of acetyl-CoA hydrolase (EC 3.1.2.1). [1-¹⁴C]Acetyl-CoA is incubated with tissue homogenates; unhydrolyzed [1-¹⁴C]acetyl-CoA is separated from the radiolabeled product, [1-¹⁴C]acetate, by adsorption to charcoal. The soluble [1-¹⁴C]acetate is measured by liquid scintillation techniques. This procedure makes it possible to measure as little as 0.2 to 0.4 nmol acetate generated per assay.

Original language	English
Pages (from-to)	244-247
Number of pages	4
Journal	Analytical Biochemistry
Volume	93
Issue number	C
DOIs	https://doi.org/10.1016/S0003-2697(79)80145-1
State	Published - 1979

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title = "Use of charcoal adsorption for the microassay of Acetyl-CoA hydrolase",

abstract = "A sensitive and rapid radiochemical micromethod is described for measuring the activity of acetyl-CoA hydrolase (EC 3.1.2.1). [1-14C]Acetyl-CoA is incubated with tissue homogenates; unhydrolyzed [1-14C]acetyl-CoA is separated from the radiolabeled product, [1-14C]acetate, by adsorption to charcoal. The soluble [1-14C]acetate is measured by liquid scintillation techniques. This procedure makes it possible to measure as little as 0.2 to 0.4 nmol acetate generated per assay.",

author = "Namboodiri, \{M. A.A.\} and Klein, \{D. C.\}",

year = "1979",

doi = "10.1016/S0003-2697(79)80145-1",

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pages = "244--247",

journal = "Analytical Biochemistry",

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T1 - Use of charcoal adsorption for the microassay of Acetyl-CoA hydrolase

AU - Namboodiri, M. A.A.

AU - Klein, D. C.

PY - 1979

Y1 - 1979

N2 - A sensitive and rapid radiochemical micromethod is described for measuring the activity of acetyl-CoA hydrolase (EC 3.1.2.1). [1-14C]Acetyl-CoA is incubated with tissue homogenates; unhydrolyzed [1-14C]acetyl-CoA is separated from the radiolabeled product, [1-14C]acetate, by adsorption to charcoal. The soluble [1-14C]acetate is measured by liquid scintillation techniques. This procedure makes it possible to measure as little as 0.2 to 0.4 nmol acetate generated per assay.

AB - A sensitive and rapid radiochemical micromethod is described for measuring the activity of acetyl-CoA hydrolase (EC 3.1.2.1). [1-14C]Acetyl-CoA is incubated with tissue homogenates; unhydrolyzed [1-14C]acetyl-CoA is separated from the radiolabeled product, [1-14C]acetate, by adsorption to charcoal. The soluble [1-14C]acetate is measured by liquid scintillation techniques. This procedure makes it possible to measure as little as 0.2 to 0.4 nmol acetate generated per assay.

UR - https://www.scopus.com/pages/publications/0018414419

U2 - 10.1016/S0003-2697(79)80145-1

DO - 10.1016/S0003-2697(79)80145-1

M3 - Article

C2 - 37774

AN - SCOPUS:0018414419

SN - 0003-2697

VL - 93

SP - 244

EP - 247

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - C

ER -

Use of charcoal adsorption for the microassay of Acetyl-CoA hydrolase

Abstract

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