Use of fluorescent microspheres to localize in vivo gene transfer injection sites

Michael A. Flynn*, Y. Vodovotz, R. Kornowski, S. Epstein, D. Gordon, J. A. Keiser

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The potential for using gene therapy to treat a variety of disease states is growing rapidly. Many vector types and delivery systems have been developed that allow the optimization of protein production levels and kinetics for a given therapeutic gene product. In cases in which a transient, localized delivery of gene product is desired, any determination of the locale of transfected tissue by non-marker genes is problematic. We describe a technique by which the use of fluorescent microspheres can help in identifying potentially transfected tissue. Adenovirus containing the gene for β-galactosidase (β-gal) was mixed with fluorescent microspheres and injected into rat skeletal muscle and porcine myocardium. The injection sites could be visualized under ultraviolet light and correlated with β-gal enzyme expression. This method is simple, inexpensive and generally useful for in vivo gene transfer experiments.

Original languageEnglish
Pages (from-to)470-476
Number of pages7
Issue number3
StatePublished - 2000
Externally publishedYes


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