Use of Plasma Metagenomic Next-generation Sequencing for Pathogen Identification in Pediatric Endocarditis

Rachel K. To; Nanda Ramchandar; Aamisha Gupta; Alice Pong; Christopher Cannavino; Jennifer Foley; Lauge Farnaes; Nicole G. Coufal

doi:10.1097/INF.0000000000003038

Use of Plasma Metagenomic Next-generation Sequencing for Pathogen Identification in Pediatric Endocarditis

Rachel K. To
, Nanda Ramchandar
, Aamisha Gupta
, Alice Pong
, Christopher Cannavino
, Jennifer Foley
, Lauge Farnaes
, Nicole G. Coufal^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Pediatric infective endocarditis incurs significant morbidity and generally occurs among children with underlying heart disease. Identification of a pathogen is critical in determining appropriate therapy. However, standard diagnostic testing has limited sensitivity. We describe a case series of children with infective endocarditis in whom plasma next-generation sequencing (Karius, Redwood, CA) identified an organism in 8 of 10 cases.

Original language	English
Pages (from-to)	486-488
Number of pages	3
Journal	Pediatric Infectious Disease Journal
Volume	40
Issue number	5
DOIs	https://doi.org/10.1097/INF.0000000000003038
State	Published - 1 May 2021

Keywords

infectious endocarditis
metagenomics
next-generation sequencing

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10.1097/INF.0000000000003038

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title = "Use of Plasma Metagenomic Next-generation Sequencing for Pathogen Identification in Pediatric Endocarditis",

abstract = "Pediatric infective endocarditis incurs significant morbidity and generally occurs among children with underlying heart disease. Identification of a pathogen is critical in determining appropriate therapy. However, standard diagnostic testing has limited sensitivity. We describe a case series of children with infective endocarditis in whom plasma next-generation sequencing (Karius, Redwood, CA) identified an organism in 8 of 10 cases.",

keywords = "infectious endocarditis, metagenomics, next-generation sequencing",

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AU - To, Rachel K.

AU - Ramchandar, Nanda

AU - Gupta, Aamisha

AU - Pong, Alice

AU - Cannavino, Christopher

AU - Foley, Jennifer

AU - Farnaes, Lauge

AU - Coufal, Nicole G.

PY - 2021/5/1

Y1 - 2021/5/1

N2 - Pediatric infective endocarditis incurs significant morbidity and generally occurs among children with underlying heart disease. Identification of a pathogen is critical in determining appropriate therapy. However, standard diagnostic testing has limited sensitivity. We describe a case series of children with infective endocarditis in whom plasma next-generation sequencing (Karius, Redwood, CA) identified an organism in 8 of 10 cases.

AB - Pediatric infective endocarditis incurs significant morbidity and generally occurs among children with underlying heart disease. Identification of a pathogen is critical in determining appropriate therapy. However, standard diagnostic testing has limited sensitivity. We describe a case series of children with infective endocarditis in whom plasma next-generation sequencing (Karius, Redwood, CA) identified an organism in 8 of 10 cases.

KW - infectious endocarditis

KW - metagenomics

KW - next-generation sequencing

UR - https://www.scopus.com/pages/publications/85104275860

U2 - 10.1097/INF.0000000000003038

DO - 10.1097/INF.0000000000003038

M3 - Article

C2 - 33410648

AN - SCOPUS:85104275860

SN - 0891-3668

VL - 40

SP - 486

EP - 488

JO - Pediatric Infectious Disease Journal

JF - Pediatric Infectious Disease Journal

IS - 5

ER -

Use of Plasma Metagenomic Next-generation Sequencing for Pathogen Identification in Pediatric Endocarditis

Abstract

Keywords

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