Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

R. J. Danaher; E. F. Petricoin; D. C. Stein

doi:10.1128/jb.176.11.3428-3432.1994

Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

R. J. Danaher
, E. F. Petricoin
, D. C. Stein^*

^*Corresponding author for this work

Research output: Contribution to journal › Comment/debate

7 Scopus citations

Abstract

The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.

Original language	English
Pages (from-to)	3428-3432
Number of pages	5
Journal	Journal of Bacteriology
Volume	176
Issue number	11
DOIs	https://doi.org/10.1128/jb.176.11.3428-3432.1994
State	Published - 1994

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10.1128/jb.176.11.3428-3432.1994

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title = "Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked",

abstract = "The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.",

author = "Danaher, \{R. J.\} and Petricoin, \{E. F.\} and Stein, \{D. C.\}",

year = "1994",

doi = "10.1128/jb.176.11.3428-3432.1994",

language = "English",

volume = "176",

pages = "3428--3432",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "11",

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TY - JOUR

T1 - Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

AU - Danaher, R. J.

AU - Petricoin, E. F.

AU - Stein, D. C.

PY - 1994

Y1 - 1994

N2 - The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.

AB - The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.

UR - https://www.scopus.com/pages/publications/0028233324

U2 - 10.1128/jb.176.11.3428-3432.1994

DO - 10.1128/jb.176.11.3428-3432.1994

M3 - Comment/debate

C2 - 8195104

AN - SCOPUS:0028233324

SN - 0021-9193

VL - 176

SP - 3428

EP - 3432

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 11

ER -

Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

Abstract

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