Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

R. J. Danaher; E. F. Petricoin; D. C. Stein

doi:10.1128/jb.176.11.3428-3432.1994

Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

R. J. Danaher, E. F. Petricoin, D. C. Stein^*

^*Corresponding author for this work

Research output: Contribution to journal › Comment/debate

7 Scopus citations

Abstract

The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.

Original language	English
Pages (from-to)	3428-3432
Number of pages	5
Journal	Journal of Bacteriology
Volume	176
Issue number	11
DOIs	https://doi.org/10.1128/jb.176.11.3428-3432.1994
State	Published - 1994
Externally published	Yes

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10.1128/jb.176.11.3428-3432.1994

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title = "Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked",

abstract = "The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.",

author = "Danaher, {R. J.} and Petricoin, {E. F.} and Stein, {D. C.}",

year = "1994",

doi = "10.1128/jb.176.11.3428-3432.1994",

language = "English",

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journal = "Journal of Bacteriology",

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publisher = "American Society for Microbiology",

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T1 - Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

AU - Danaher, R. J.

AU - Petricoin, E. F.

AU - Stein, D. C.

PY - 1994

Y1 - 1994

N2 - The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.

AB - The Neisseria gonorrhoeae lipooligosaccharide biosynthetic gene lsi-1 is preceded by a 281-bp non-protein-encoding sequence, lsi-3, that contains two pairs of inverted repeats. Gonococcal chromosomal lsi-xylE gene fusions were generated to measure the effect of the secondary structure on transcriptional attenuation. The data obtained indicate that lsi-3 and lsi-1 are not transcriptionally linked and therefore that lsi-3 is not involved in the regulation of lsi-1.

UR - http://www.scopus.com/inward/record.url?scp=0028233324&partnerID=8YFLogxK

U2 - 10.1128/jb.176.11.3428-3432.1994

DO - 10.1128/jb.176.11.3428-3432.1994

M3 - Comment/debate

C2 - 8195104

AN - SCOPUS:0028233324

SN - 0021-9193

VL - 176

SP - 3428

EP - 3432

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 11

ER -

Use of xylE fusions to demonstrate that lsi-1, a Neisseria gonorrheae lipooligosaccharide biosynthetic gene, and lsi-3 are not transcriptionally linked

Abstract

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