@inbook{31142c79712b472db56df0a59471fa19,
title = "Using C. elegans to identify the protease targets of serpins in vivo",
abstract = "Most serpins inhibit serine and/or cysteine proteases, and their inhibitory activities are usually defined in vitro. However, the physiological protease targets of most serpins are unknown despite many years of research. This may be due to the rapid degradation of the inactive serpin:protease complexes and/or the conditions under which the serpin inhibits the protease. The model organism Caenorhabditis elegans is an ideal system for identifying protease targets due to powerful forward and reverse genetics, as well as the ease of creating transgenic animals. Using combinatorial approaches of genetics and biochemistry in C. elegans, the true in vivo protease targets of the endogenous serpins can be elucidated.",
keywords = "Caenorhabditis elegans, Mass spectrometry, RNAi, Serpin, Tandem affinity purification",
author = "Bhatia, {Sangeeta R.} and Miedel, {Mark T.} and Chotoo, {Cavita K.} and Graf, {Nathan J.} and Hood, {Brian L.} and Conrads, {Thomas P.} and Silverman, {Gary A.} and Luke, {Cliff J.}",
note = "Funding Information: The authors would like to thank the entire C. elegans research community for their continued help and support for over a decade. This work was supported, in whole or in part, by National Institutes of Health Grants DK079806 and DK081422 (to G. A. S.). S. R. B. is supported through grant HD052892 Department of Human Health Services, Public Health Services. M. T. M. is supported by an individual NRSA F32DK086112 from the National Institute of Diabetes and Digestive and Kidney Diseases. C. K. C. is a graduate student, part of the Cell Biology and Molecular Physiology Graduate Program (CBMP) of the University of Pittsburgh and supported through the CBMP teaching fellowship.",
year = "2011",
doi = "10.1016/B978-0-12-386471-0.00014-6",
language = "English",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "283--299",
booktitle = "Methods in Enzymology",
}