Variations of CHROMagar Acinetobacter to detect imipenem-resistant Acinetobacter baumannii-calcoaceticus complex

Alice Barsoumian, Tatjana Calvano, Ana E. Markelz, Ryan Cassidy, Clinton K. Murray, Miriam L. Beckius, Katrin Mende, Kevin S. Akers*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Background: Acinetobacter baumannii-calcoaceticus complex (ABC) isolates are often multidrug-resistant, including to carbapenems. Chromogenic media can facilitate the rapid detection of Gram-negative bacteria, often with the addition of supplements to a base chromogenic medium to detect resistance. We examined various combinations of available media to detect imipenem resistance among 107 ABC clinical isolates. Methods: CHROMagar Orientation, CHROMagar KPC, and CHROMagar Acinetobacter, by itself, with Acinetobacter supplement, with KPC supplement, or CHROMagar Acinetobacter with increasing concentrations (1, 2.5, and 5 ml/l) of a new CR102 supplement, were examined. Results: Sensitivity for the detection of isolates was high (> 98%) for all formulations. Specificity was high for CHROMagar Acinetobacter with 2.5 ml/l and 5 ml/l of the CR102 supplement, at 95.3% and 97.7%, respectively, with positive predictive values of 97% and 98.5%. Negative predictive values of these 2 formulations were 100%. Conclusions: CHROMagar Acinetobacter with the addition of the CR102 supplement at 2.5 ml/l and 5ml/l is highly sensitive and specific for the detection of imipenem-resistant ABC, and may be useful for the rapid detection of imipenem-resistant ABC in clinical samples.

Original languageEnglish
Pages (from-to)446-452
Number of pages7
JournalScandinavian Journal of Infectious Diseases
Issue number6
StatePublished - Jun 2013


  • Acinetobacter baumannii-calcoaceticus
  • CHROMagar
  • Carbapenem
  • Complex
  • MDR


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