Vascular endothelial growth factor in porcine-derived extracellular matrix

J. P. Hodde, R. D. Record, H. A. Liang, S. F. Badylak*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

311 Scopus citations

Abstract

An extracellular matrix (ECM) derived from the submucosa of the porcine small intestine (SIS) has been shown to induce angiogenesis and host tissue remodeling when used as a xenogeneic bioscaffold in animal models of wound repair. In the present study, we compared the in vitro effects of SIS ECM extracts to several purified angiogenic growth factors on human dermal microvascular endothelial cell (HMEC) growth patterns. The SIS ECM was shown to induce tube formation from HMEC in a three-dimensional fibrin-based angiogenesis assay in a manner similar to that caused by the addition of vascular endothelial growth factor (VEGF). This tube formation was blocked in the presence of anti-VEGF neutralizing antibody. Western blots and ELISA procedures showed that the SIS ECM contains as much as 0.77 ng VEGF / g SIS. The closely related endothelial cell mitogen, platelet-derived growth factor (PDGF), was not detectable in the SIS extracts. We conclude that VEGF is present in the SIS extracellular matrix. The role of VEGF in SIS-induced wound repair remains unknown, but its presence in the ECM makes it a possible contributor to the angiogenic effect of SIS when this ECM is used as a tissue repair scaffold in animal models of wound repair.

Original languageEnglish
Pages (from-to)11-24
Number of pages14
JournalEndothelium: Journal of Endothelial Cell Research
Volume8
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Angiogenesis
  • Extracellular matrix
  • Platelet-derived growth factor
  • Small intestinal submucosa
  • Vascular endothelial growth factor

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