TY - JOUR
T1 - Vascular endothelial growth factor in porcine-derived extracellular matrix
AU - Hodde, J. P.
AU - Record, R. D.
AU - Liang, H. A.
AU - Badylak, S. F.
N1 - Funding Information:
The authors gratefully acknowledge the technical assistance of Jennifei-K. Savaiano. Financial support was provided by the P L I K ~ULnIiv~e rsity TRASK Fund ancl Cook Biotech, Inc.
PY - 2001
Y1 - 2001
N2 - An extracellular matrix (ECM) derived from the submucosa of the porcine small intestine (SIS) has been shown to induce angiogenesis and host tissue remodeling when used as a xenogeneic bioscaffold in animal models of wound repair. In the present study, we compared the in vitro effects of SIS ECM extracts to several purified angiogenic growth factors on human dermal microvascular endothelial cell (HMEC) growth patterns. The SIS ECM was shown to induce tube formation from HMEC in a three-dimensional fibrin-based angiogenesis assay in a manner similar to that caused by the addition of vascular endothelial growth factor (VEGF). This tube formation was blocked in the presence of anti-VEGF neutralizing antibody. Western blots and ELISA procedures showed that the SIS ECM contains as much as 0.77 ng VEGF / g SIS. The closely related endothelial cell mitogen, platelet-derived growth factor (PDGF), was not detectable in the SIS extracts. We conclude that VEGF is present in the SIS extracellular matrix. The role of VEGF in SIS-induced wound repair remains unknown, but its presence in the ECM makes it a possible contributor to the angiogenic effect of SIS when this ECM is used as a tissue repair scaffold in animal models of wound repair.
AB - An extracellular matrix (ECM) derived from the submucosa of the porcine small intestine (SIS) has been shown to induce angiogenesis and host tissue remodeling when used as a xenogeneic bioscaffold in animal models of wound repair. In the present study, we compared the in vitro effects of SIS ECM extracts to several purified angiogenic growth factors on human dermal microvascular endothelial cell (HMEC) growth patterns. The SIS ECM was shown to induce tube formation from HMEC in a three-dimensional fibrin-based angiogenesis assay in a manner similar to that caused by the addition of vascular endothelial growth factor (VEGF). This tube formation was blocked in the presence of anti-VEGF neutralizing antibody. Western blots and ELISA procedures showed that the SIS ECM contains as much as 0.77 ng VEGF / g SIS. The closely related endothelial cell mitogen, platelet-derived growth factor (PDGF), was not detectable in the SIS extracts. We conclude that VEGF is present in the SIS extracellular matrix. The role of VEGF in SIS-induced wound repair remains unknown, but its presence in the ECM makes it a possible contributor to the angiogenic effect of SIS when this ECM is used as a tissue repair scaffold in animal models of wound repair.
KW - Angiogenesis
KW - Extracellular matrix
KW - Platelet-derived growth factor
KW - Small intestinal submucosa
KW - Vascular endothelial growth factor
UR - http://www.scopus.com/inward/record.url?scp=0035007846&partnerID=8YFLogxK
U2 - 10.3109/10623320109063154
DO - 10.3109/10623320109063154
M3 - Article
C2 - 11409848
AN - SCOPUS:0035007846
SN - 1062-3329
VL - 8
SP - 11
EP - 24
JO - Endothelium: Journal of Endothelial Cell Research
JF - Endothelium: Journal of Endothelial Cell Research
IS - 1
ER -