Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19

Robin McKenzie; Chad K. Porter; Joyce A. Cantrell; Barbara DeNearing; Aisling O'Dowd; Shannon L. Grahek; Stephanie A. Sincock; Colleen Woods; Peter Sebeny; David A. Sack; David R. Tribble; A. Louis Bourgeois; Stephen J. Savarino

doi:10.1093/infdis/jir220

Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19

Robin McKenzie, Chad K. Porter, Joyce A. Cantrell, Barbara DeNearing, Aisling O'Dowd, Shannon L. Grahek, Stephanie A. Sincock, Colleen Woods, Peter Sebeny, David A. Sack, David R. Tribble, A. Louis Bourgeois, Stephen J. Savarino

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21 Scopus citations

Abstract

Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 10⁹ organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 10⁹ organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.

Original language	English
Pages (from-to)	60-64
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	204
Issue number	1
DOIs	https://doi.org/10.1093/infdis/jir220
State	Published - 1 Jul 2011

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McKenzie, R., Porter, C. K., Cantrell, J. A., DeNearing, B., O'Dowd, A., Grahek, S. L., Sincock, S. A., Woods, C., Sebeny, P., Sack, D. A., Tribble, D. R., Bourgeois, A. L., & Savarino, S. J. (2011). Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19. Journal of Infectious Diseases, 204(1), 60-64. https://doi.org/10.1093/infdis/jir220

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title = "Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19",

abstract = "Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 109 organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 109 organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.",

author = "Robin McKenzie and Porter, {Chad K.} and Cantrell, {Joyce A.} and Barbara DeNearing and Aisling O'Dowd and Grahek, {Shannon L.} and Sincock, {Stephanie A.} and Colleen Woods and Peter Sebeny and Sack, {David A.} and Tribble, {David R.} and Bourgeois, {A. Louis} and Savarino, {Stephen J.}",

note = "Funding Information: This work was supported by the Peer-Reviewed Medical Research Program (W81XWH-04-1-0067 to SJS) and by Johns Hopkins University School of Medicine General Clinical Research Center (M01-RR00052 from the National Center for Research Resources, National Institutes of Health).",

year = "2011",

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doi = "10.1093/infdis/jir220",

language = "English",

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pages = "60--64",

journal = "Journal of Infectious Diseases",

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McKenzie, R, Porter, CK, Cantrell, JA, DeNearing, B, O'Dowd, A, Grahek, SL, Sincock, SA, Woods, C, Sebeny, P, Sack, DA, Tribble, DR, Bourgeois, AL & Savarino, SJ 2011, 'Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19', Journal of Infectious Diseases, vol. 204, no. 1, pp. 60-64. https://doi.org/10.1093/infdis/jir220

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T1 - Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19

AU - McKenzie, Robin

AU - Porter, Chad K.

AU - Cantrell, Joyce A.

AU - DeNearing, Barbara

AU - O'Dowd, Aisling

AU - Grahek, Shannon L.

AU - Sincock, Stephanie A.

AU - Woods, Colleen

AU - Sebeny, Peter

AU - Sack, David A.

AU - Tribble, David R.

AU - Bourgeois, A. Louis

AU - Savarino, Stephen J.

N1 - Funding Information: This work was supported by the Peer-Reviewed Medical Research Program (W81XWH-04-1-0067 to SJS) and by Johns Hopkins University School of Medicine General Clinical Research Center (M01-RR00052 from the National Center for Research Resources, National Institutes of Health).

PY - 2011/7/1

Y1 - 2011/7/1

N2 - Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 109 organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 109 organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.

AB - Human challenges with enterotoxigenic Escherichia coli (ETEC) have broadened our understanding of this important enteropathogen. We report findings from the first challenge studies using ETEC-expressing colonization factor fimbria CS17 and CS19. LSN03-016011/A (LT, CS17) elicited a dose-dependent effect, with the upper dose (6 × 109 organisms) causing diarrhea in 88% of recipients. WS0115A (LTSTp, CS19) also showed a dose response, with a 44% diarrhea rate at 9 × 109 organisms. Both strains elicited homologous antifimbrial and anti-LT antibody seroconversion. These studies establish the relative pathogenicity of ETEC expressing newer class 5 fimbriae and suggest suitability of the LT|CS17-ETEC challenge model for interventional trials.

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Volunteer challenge with enterotoxigenic Escherichia coli that express intestinal colonization factor fimbriae CS17 and CS19

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