VSV replication in normal and transformed T cells, an assay for T suppressor cell activation

Bonita J. Rup; David W. Scott

doi:10.1016/0008-8749(87)90019-0

VSV replication in normal and transformed T cells, an assay for T suppressor cell activation

Bonita J. Rup, David W. Scott^*

^*Corresponding author for this work

Medicine

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

An infectious center viral plaque assay has been utilized to quantitate activated T suppressor (Ts) cells. This assay is based on two observations. Namely, resting T cells do not serve as good replicative hosts for many viruses, including vesicular stomatitis virus (VSV), and that Ts cells can be enriched by their ability to bind to antigen-coated dishes. Our data show that Ts cells specific for either the TNP hapten or for dextran will replicate VSV upon antigenic and/or mitogenic activation, whereas resting Ts and hapten-specific B cells are less efficient in this process. This system will now allow the direct quantitation of Ts cells and their activation properties.

Original language	English
Pages (from-to)	190-194
Number of pages	5
Journal	Cellular Immunology
Volume	104
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(87)90019-0
State	Published - Jan 1987

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title = "VSV replication in normal and transformed T cells, an assay for T suppressor cell activation",

abstract = "An infectious center viral plaque assay has been utilized to quantitate activated T suppressor (Ts) cells. This assay is based on two observations. Namely, resting T cells do not serve as good replicative hosts for many viruses, including vesicular stomatitis virus (VSV), and that Ts cells can be enriched by their ability to bind to antigen-coated dishes. Our data show that Ts cells specific for either the TNP hapten or for dextran will replicate VSV upon antigenic and/or mitogenic activation, whereas resting Ts and hapten-specific B cells are less efficient in this process. This system will now allow the direct quantitation of Ts cells and their activation properties.",

author = "Rup, {Bonita J.} and Scott, {David W.}",

note = "Funding Information: {\textquoteright} Supported by USPHS Grant CA-36 107 and funds from the Cancer Research Institute of New York. * Present address: Monsanto Chemical Co., Health Care Div., 700 Chesterfield Village Parkway, Chesterfield, MO. 63 198. {\textquoteright} To whom correspondence should be addressed.",

year = "1987",

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doi = "10.1016/0008-8749(87)90019-0",

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T1 - VSV replication in normal and transformed T cells, an assay for T suppressor cell activation

AU - Rup, Bonita J.

AU - Scott, David W.

N1 - Funding Information: ’ Supported by USPHS Grant CA-36 107 and funds from the Cancer Research Institute of New York. * Present address: Monsanto Chemical Co., Health Care Div., 700 Chesterfield Village Parkway, Chesterfield, MO. 63 198. ’ To whom correspondence should be addressed.

PY - 1987/1

Y1 - 1987/1

N2 - An infectious center viral plaque assay has been utilized to quantitate activated T suppressor (Ts) cells. This assay is based on two observations. Namely, resting T cells do not serve as good replicative hosts for many viruses, including vesicular stomatitis virus (VSV), and that Ts cells can be enriched by their ability to bind to antigen-coated dishes. Our data show that Ts cells specific for either the TNP hapten or for dextran will replicate VSV upon antigenic and/or mitogenic activation, whereas resting Ts and hapten-specific B cells are less efficient in this process. This system will now allow the direct quantitation of Ts cells and their activation properties.

AB - An infectious center viral plaque assay has been utilized to quantitate activated T suppressor (Ts) cells. This assay is based on two observations. Namely, resting T cells do not serve as good replicative hosts for many viruses, including vesicular stomatitis virus (VSV), and that Ts cells can be enriched by their ability to bind to antigen-coated dishes. Our data show that Ts cells specific for either the TNP hapten or for dextran will replicate VSV upon antigenic and/or mitogenic activation, whereas resting Ts and hapten-specific B cells are less efficient in this process. This system will now allow the direct quantitation of Ts cells and their activation properties.

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VSV replication in normal and transformed T cells, an assay for T suppressor cell activation

Abstract

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