Wheat bran an inexpensive substrate for production of Lactic acid in solid state fermentation by Lactobacillus amylophilus GV6 - Optimization of fermentation conditions

Lactic acid production by an amylolytic bacterium Lactobacillus amylophilus GV6 is studied using wheat bran in solid state fermentation. Conditions of solid state fermentations are optimized. Moisture content 83 per cent, at 37°C, pH 6.75, inoculum size 3.5 mL and incubation period of 5 d were found to be optimum. Different brans of pigeon pea, green gram, black gram and corn fibre are also tested in addition to wheat bran. Wheat bran is found to be the best as solid support and substrate among all brans tested.