Whole-genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011

Denis K. Byarugaba; Bernard Erima; Monica Millard; Hannah Kibuuka; Luswa Lkwago; Josephine Bwogi; Derrick Mimbe; Jocelyn B. Kiconco; Titus Tugume; Edison A. Mworozi; Jasmine Turner; Pamela P. Mckenzie; Richard R.J. Webby; Robert G. Webster; Charlotte Foret; Mariette F. Ducatez; Rodney Coldren; Fred Wabwire-Mangen; Scott Krauss

doi:10.1111/irv.12401

Whole-genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011

Denis K. Byarugaba^*, Bernard Erima, Monica Millard, Hannah Kibuuka, Luswa Lkwago, Josephine Bwogi, Derrick Mimbe, Jocelyn B. Kiconco, Titus Tugume, Edison A. Mworozi, Jasmine Turner, Pamela P. Mckenzie, Richard R.J. Webby, Robert G. Webster, Charlotte Foret, Mariette F. Ducatez, Rodney Coldren, Fred Wabwire-Mangen, Scott Krauss

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

We report a whole-genome analysis of 19 influenza A(H1N1)pdm09 isolates from four Ugandan hospitals between 2009 and 2011. The isolates differed from the vaccine strain A/California/07/2009 by three amino acid substitutions P100S, S220T, and I338V in the hemagglutinin and by two amino acid substitutions V106I and N248D in the neuraminidase proteins with consistent mutations in all gene segments distinguishing isolates from the 2009/2010 to 2010/2011 seasons. Phylogenetic analysis showed low genetic evolution, with genetic distances of 0%–1.3% and 0.1%–1.6% for HA and NA genes, respectively. The amino acid substitutions did not lead to antigenic differences from the reference strains.

Original language	English
Pages (from-to)	486-492
Number of pages	7
Journal	Influenza and other Respiratory Viruses
Volume	10
Issue number	6
DOIs	https://doi.org/10.1111/irv.12401
State	Published - 1 Nov 2016
Externally published	Yes

Keywords

Uganda
influenza A(H1N1)pdm09
whole-genome sequencing

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10.1111/irv.12401

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Byarugaba, D. K., Erima, B., Millard, M., Kibuuka, H., Lkwago, L., Bwogi, J., Mimbe, D., Kiconco, J. B., Tugume, T., Mworozi, E. A., Turner, J., Mckenzie, P. P., Webby, R. R. J., Webster, R. G., Foret, C., Ducatez, M. F., Coldren, R., Wabwire-Mangen, F., & Krauss, S. (2016). Whole-genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011. Influenza and other Respiratory Viruses, 10(6), 486-492. https://doi.org/10.1111/irv.12401

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title = "Whole-genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011",

abstract = "We report a whole-genome analysis of 19 influenza A(H1N1)pdm09 isolates from four Ugandan hospitals between 2009 and 2011. The isolates differed from the vaccine strain A/California/07/2009 by three amino acid substitutions P100S, S220T, and I338V in the hemagglutinin and by two amino acid substitutions V106I and N248D in the neuraminidase proteins with consistent mutations in all gene segments distinguishing isolates from the 2009/2010 to 2010/2011 seasons. Phylogenetic analysis showed low genetic evolution, with genetic distances of 0%–1.3% and 0.1%–1.6% for HA and NA genes, respectively. The amino acid substitutions did not lead to antigenic differences from the reference strains.",

keywords = "Uganda, influenza A(H1N1)pdm09, whole-genome sequencing",

author = "Byarugaba, {Denis K.} and Bernard Erima and Monica Millard and Hannah Kibuuka and Luswa Lkwago and Josephine Bwogi and Derrick Mimbe and Kiconco, {Jocelyn B.} and Titus Tugume and Mworozi, {Edison A.} and Jasmine Turner and Mckenzie, {Pamela P.} and Webby, {Richard R.J.} and Webster, {Robert G.} and Charlotte Foret and Ducatez, {Mariette F.} and Rodney Coldren and Fred Wabwire-Mangen and Scott Krauss",

note = "Publisher Copyright: {\textcopyright} 2016 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.",

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Byarugaba, DK, Erima, B, Millard, M, Kibuuka, H, Lkwago, L, Bwogi, J, Mimbe, D, Kiconco, JB, Tugume, T, Mworozi, EA, Turner, J, Mckenzie, PP, Webby, RRJ, Webster, RG, Foret, C, Ducatez, MF, Coldren, R, Wabwire-Mangen, F & Krauss, S 2016, 'Whole-genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011', Influenza and other Respiratory Viruses, vol. 10, no. 6, pp. 486-492. https://doi.org/10.1111/irv.12401

TY - JOUR

T1 - Whole-genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011

AU - Byarugaba, Denis K.

AU - Erima, Bernard

AU - Millard, Monica

AU - Kibuuka, Hannah

AU - Lkwago, Luswa

AU - Bwogi, Josephine

AU - Mimbe, Derrick

AU - Kiconco, Jocelyn B.

AU - Tugume, Titus

AU - Mworozi, Edison A.

AU - Turner, Jasmine

AU - Mckenzie, Pamela P.

AU - Webby, Richard R.J.

AU - Webster, Robert G.

AU - Foret, Charlotte

AU - Ducatez, Mariette F.

AU - Coldren, Rodney

AU - Wabwire-Mangen, Fred

AU - Krauss, Scott

PY - 2016/11/1

Y1 - 2016/11/1

N2 - We report a whole-genome analysis of 19 influenza A(H1N1)pdm09 isolates from four Ugandan hospitals between 2009 and 2011. The isolates differed from the vaccine strain A/California/07/2009 by three amino acid substitutions P100S, S220T, and I338V in the hemagglutinin and by two amino acid substitutions V106I and N248D in the neuraminidase proteins with consistent mutations in all gene segments distinguishing isolates from the 2009/2010 to 2010/2011 seasons. Phylogenetic analysis showed low genetic evolution, with genetic distances of 0%–1.3% and 0.1%–1.6% for HA and NA genes, respectively. The amino acid substitutions did not lead to antigenic differences from the reference strains.

AB - We report a whole-genome analysis of 19 influenza A(H1N1)pdm09 isolates from four Ugandan hospitals between 2009 and 2011. The isolates differed from the vaccine strain A/California/07/2009 by three amino acid substitutions P100S, S220T, and I338V in the hemagglutinin and by two amino acid substitutions V106I and N248D in the neuraminidase proteins with consistent mutations in all gene segments distinguishing isolates from the 2009/2010 to 2010/2011 seasons. Phylogenetic analysis showed low genetic evolution, with genetic distances of 0%–1.3% and 0.1%–1.6% for HA and NA genes, respectively. The amino acid substitutions did not lead to antigenic differences from the reference strains.

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JO - Influenza and other Respiratory Viruses

JF - Influenza and other Respiratory Viruses

IS - 6

ER -

Whole-genome analysis of influenza A(H1N1)pdm09 viruses isolated in Uganda from 2009 to 2011

Abstract

Keywords

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