Whole genome sequencing and antimicrobial resistance of staphylococcus aureus from surgical site infections in Ghana

Beverly Egyir; Jeannette Bentum; Naiki Attram; Anne Fox; Noah Obeng-Nkrumah; Labi Appiah-Korang; Eric Behene; Selassie Kumordjie; Clara Yeboah; Bright Agbodzi; Ronald Essah Bentil; Rhodalyn Tagoe; Blessing Kofi Adu Tabi; Felicia Owusu; Nicholas T.K.D. Dayie; Eric S. Donkor; Josephine Nsaful; Kwaku Asah-Opoku; Edward Nyarko; Edward Asumanu; Anders Rhod Larsen; David M. Wolfe; Andrew G. Letizia

doi:10.3390/pathogens10020196

Whole genome sequencing and antimicrobial resistance of staphylococcus aureus from surgical site infections in Ghana

Beverly Egyir^*, Jeannette Bentum, Naiki Attram, Anne Fox, Noah Obeng-Nkrumah, Labi Appiah-Korang, Eric Behene, Selassie Kumordjie, Clara Yeboah, Bright Agbodzi, Ronald Essah Bentil, Rhodalyn Tagoe, Blessing Kofi Adu Tabi, Felicia Owusu, Nicholas T.K.D. Dayie, Eric S. Donkor, Josephine Nsaful, Kwaku Asah-Opoku, Edward Nyarko, Edward AsumanuAnders Rhod Larsen, David M. Wolfe, Andrew G. Letizia

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Staphylococcus aureus (S. aureus) is a common cause of surgical site infections (SSIs) globally. Data on the occurrence of methicillin-susceptible S. aureus (MSSA) as well as methicillin-resistant S. aureus (MRSA) among patients with surgical site infections (SSIs) in sub-Saharan African are scarce. We characterized S. aureus from SSIs in Ghana using molecular methods and antimicrobial susceptibility testing (AST). Wound swabs or aspirate samples were collected from subjects with SSIs. S. aureus was identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS); AST was performed by Kirby-Bauer disk diffusion, and results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guideline. Detection of spa, mecA, and pvl genes was performed by polymerase chain reaction (PCR). Whole-genome sequencing (WGS) was done using the Illumina MiSeq platform. Samples were collected from 112 subjects, with 13 S. aureus isolates recovered. Of these, 92% were sensitive to co-trimoxazole, 77% to clindamycin, and 54% to erythromycin. Multi-drug resistance was detected in 5 (38%) isolates. The four mecA gene-positive MRSA isolates detected belonged to ST152 (n = 3) and ST5 (n = 1). In total, 62% of the isolates were positive for the Panton-Valentine leukocidin (pvl) toxin gene. This study reports, for the first time, a pvl-positive ST152-t355 MRSA clone from SSIs in Ghana. The occurrence of multi-drug-resistant S. aureus epidemic clones suggests that continuous surveillance is required to monitor the spread and resistance trends of S. aureus in hospital settings in the country.

Original language	English
Article number	196
Pages (from-to)	1-13
Number of pages	13
Journal	Pathogens
Volume	10
Issue number	2
DOIs	https://doi.org/10.3390/pathogens10020196
State	Published - Feb 2021
Externally published	Yes

Keywords

Africa
Mrsa
Surgical site infections
Whole-genome sequencing

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10.3390/pathogens10020196

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Egyir, B., Bentum, J., Attram, N., Fox, A., Obeng-Nkrumah, N., Appiah-Korang, L., Behene, E., Kumordjie, S., Yeboah, C., Agbodzi, B., Bentil, R. E., Tagoe, R., Tabi, B. K. A., Owusu, F., Dayie, N. T. K. D., Donkor, E. S., Nsaful, J., Asah-Opoku, K., Nyarko, E., ... Letizia, A. G. (2021). Whole genome sequencing and antimicrobial resistance of staphylococcus aureus from surgical site infections in Ghana. Pathogens, 10(2), 1-13. Article 196. https://doi.org/10.3390/pathogens10020196

@article{1ec0cab6b5234e92b0509ea0a4b0af87,

title = "Whole genome sequencing and antimicrobial resistance of staphylococcus aureus from surgical site infections in Ghana",

abstract = "Staphylococcus aureus (S. aureus) is a common cause of surgical site infections (SSIs) globally. Data on the occurrence of methicillin-susceptible S. aureus (MSSA) as well as methicillin-resistant S. aureus (MRSA) among patients with surgical site infections (SSIs) in sub-Saharan African are scarce. We characterized S. aureus from SSIs in Ghana using molecular methods and antimicrobial susceptibility testing (AST). Wound swabs or aspirate samples were collected from subjects with SSIs. S. aureus was identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS); AST was performed by Kirby-Bauer disk diffusion, and results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guideline. Detection of spa, mecA, and pvl genes was performed by polymerase chain reaction (PCR). Whole-genome sequencing (WGS) was done using the Illumina MiSeq platform. Samples were collected from 112 subjects, with 13 S. aureus isolates recovered. Of these, 92% were sensitive to co-trimoxazole, 77% to clindamycin, and 54% to erythromycin. Multi-drug resistance was detected in 5 (38%) isolates. The four mecA gene-positive MRSA isolates detected belonged to ST152 (n = 3) and ST5 (n = 1). In total, 62% of the isolates were positive for the Panton-Valentine leukocidin (pvl) toxin gene. This study reports, for the first time, a pvl-positive ST152-t355 MRSA clone from SSIs in Ghana. The occurrence of multi-drug-resistant S. aureus epidemic clones suggests that continuous surveillance is required to monitor the spread and resistance trends of S. aureus in hospital settings in the country.",

keywords = "Africa, Mrsa, Surgical site infections, Whole-genome sequencing",

author = "Beverly Egyir and Jeannette Bentum and Naiki Attram and Anne Fox and Noah Obeng-Nkrumah and Labi Appiah-Korang and Eric Behene and Selassie Kumordjie and Clara Yeboah and Bright Agbodzi and Bentil, {Ronald Essah} and Rhodalyn Tagoe and Tabi, {Blessing Kofi Adu} and Felicia Owusu and Dayie, {Nicholas T.K.D.} and Donkor, {Eric S.} and Josephine Nsaful and Kwaku Asah-Opoku and Edward Nyarko and Edward Asumanu and Larsen, {Anders Rhod} and Wolfe, {David M.} and Letizia, {Andrew G.}",

note = "Publisher Copyright: {\textcopyright} 2021 by the author. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = feb,

doi = "10.3390/pathogens10020196",

language = "English",

volume = "10",

pages = "1--13",

journal = "Pathogens",

issn = "2076-0817",

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Egyir, B, Bentum, J, Attram, N, Fox, A, Obeng-Nkrumah, N, Appiah-Korang, L, Behene, E, Kumordjie, S, Yeboah, C, Agbodzi, B, Bentil, RE, Tagoe, R, Tabi, BKA, Owusu, F, Dayie, NTKD, Donkor, ES, Nsaful, J, Asah-Opoku, K, Nyarko, E, Asumanu, E, Larsen, AR, Wolfe, DM & Letizia, AG 2021, 'Whole genome sequencing and antimicrobial resistance of staphylococcus aureus from surgical site infections in Ghana', Pathogens, vol. 10, no. 2, 196, pp. 1-13. https://doi.org/10.3390/pathogens10020196

TY - JOUR

T1 - Whole genome sequencing and antimicrobial resistance of staphylococcus aureus from surgical site infections in Ghana

AU - Egyir, Beverly

AU - Bentum, Jeannette

AU - Attram, Naiki

AU - Fox, Anne

AU - Obeng-Nkrumah, Noah

AU - Appiah-Korang, Labi

AU - Behene, Eric

AU - Kumordjie, Selassie

AU - Yeboah, Clara

AU - Agbodzi, Bright

AU - Bentil, Ronald Essah

AU - Tagoe, Rhodalyn

AU - Tabi, Blessing Kofi Adu

AU - Owusu, Felicia

AU - Dayie, Nicholas T.K.D.

AU - Donkor, Eric S.

AU - Nsaful, Josephine

AU - Asah-Opoku, Kwaku

AU - Nyarko, Edward

AU - Asumanu, Edward

AU - Larsen, Anders Rhod

AU - Wolfe, David M.

AU - Letizia, Andrew G.

PY - 2021/2

Y1 - 2021/2

N2 - Staphylococcus aureus (S. aureus) is a common cause of surgical site infections (SSIs) globally. Data on the occurrence of methicillin-susceptible S. aureus (MSSA) as well as methicillin-resistant S. aureus (MRSA) among patients with surgical site infections (SSIs) in sub-Saharan African are scarce. We characterized S. aureus from SSIs in Ghana using molecular methods and antimicrobial susceptibility testing (AST). Wound swabs or aspirate samples were collected from subjects with SSIs. S. aureus was identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS); AST was performed by Kirby-Bauer disk diffusion, and results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guideline. Detection of spa, mecA, and pvl genes was performed by polymerase chain reaction (PCR). Whole-genome sequencing (WGS) was done using the Illumina MiSeq platform. Samples were collected from 112 subjects, with 13 S. aureus isolates recovered. Of these, 92% were sensitive to co-trimoxazole, 77% to clindamycin, and 54% to erythromycin. Multi-drug resistance was detected in 5 (38%) isolates. The four mecA gene-positive MRSA isolates detected belonged to ST152 (n = 3) and ST5 (n = 1). In total, 62% of the isolates were positive for the Panton-Valentine leukocidin (pvl) toxin gene. This study reports, for the first time, a pvl-positive ST152-t355 MRSA clone from SSIs in Ghana. The occurrence of multi-drug-resistant S. aureus epidemic clones suggests that continuous surveillance is required to monitor the spread and resistance trends of S. aureus in hospital settings in the country.

AB - Staphylococcus aureus (S. aureus) is a common cause of surgical site infections (SSIs) globally. Data on the occurrence of methicillin-susceptible S. aureus (MSSA) as well as methicillin-resistant S. aureus (MRSA) among patients with surgical site infections (SSIs) in sub-Saharan African are scarce. We characterized S. aureus from SSIs in Ghana using molecular methods and antimicrobial susceptibility testing (AST). Wound swabs or aspirate samples were collected from subjects with SSIs. S. aureus was identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS); AST was performed by Kirby-Bauer disk diffusion, and results were interpreted according to the Clinical and Laboratory Standards Institute (CLSI) guideline. Detection of spa, mecA, and pvl genes was performed by polymerase chain reaction (PCR). Whole-genome sequencing (WGS) was done using the Illumina MiSeq platform. Samples were collected from 112 subjects, with 13 S. aureus isolates recovered. Of these, 92% were sensitive to co-trimoxazole, 77% to clindamycin, and 54% to erythromycin. Multi-drug resistance was detected in 5 (38%) isolates. The four mecA gene-positive MRSA isolates detected belonged to ST152 (n = 3) and ST5 (n = 1). In total, 62% of the isolates were positive for the Panton-Valentine leukocidin (pvl) toxin gene. This study reports, for the first time, a pvl-positive ST152-t355 MRSA clone from SSIs in Ghana. The occurrence of multi-drug-resistant S. aureus epidemic clones suggests that continuous surveillance is required to monitor the spread and resistance trends of S. aureus in hospital settings in the country.

KW - Africa

KW - Mrsa

KW - Surgical site infections

KW - Whole-genome sequencing

UR - http://www.scopus.com/inward/record.url?scp=85101581034&partnerID=8YFLogxK

U2 - 10.3390/pathogens10020196

DO - 10.3390/pathogens10020196

M3 - Article

AN - SCOPUS:85101581034

SN - 2076-0817

VL - 10

SP - 1

EP - 13

JO - Pathogens

JF - Pathogens

IS - 2

M1 - 196

ER -

Whole genome sequencing and antimicrobial resistance of staphylococcus aureus from surgical site infections in Ghana

Abstract

Keywords

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