TY - JOUR
T1 - Whole genome sequencing of diverse Shiga toxin-producing and non-producing Escherichia coli strains reveals a variety of virulence and novel antibiotic resistance plasmids
AU - Losada, Liliana
AU - DebRoy, Chitrita
AU - Radune, Diana
AU - Kim, Maria
AU - Sanka, Ravi
AU - Brinkac, Lauren
AU - Kariyawasam, Subhashinie
AU - Shelton, Daniel
AU - Fratamico, Pina M.
AU - Kapur, Vivek
AU - Feng, Peter C.H.
N1 - Publisher Copyright:
© 2016.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - The genomes of a diverse set of Escherichia coli, including many Shiga toxin-producing strains of various serotypes were determined. A total of 39 plasmids were identified among these strains, and many carried virulence or putative virulence genes of Shiga toxin-producing E. coli strains, virulence genes for other pathogenic E. coli groups, and some had combinations of these genes. Among the novel plasmids identified were eight that carried resistance genes to aminoglycosides, carbapenems, penicillins, cephalosporins, chloramphenicol, dihydrofolate reductase inhibitors, sulfonamides, tetracyclines and resistance to heavy metals. Two of the plasmids carried six of these resistance genes and two novel IncHI2 plasmids were also identified. The results of this study showed that plasmids carrying diverse resistance and virulence genes of various pathogenic E. coli groups can be found in E. coli strains and serotypes regardless of the isolate's source and therefore, is consistent with the premise that these mobile elements carrying these traits may be broadly disseminated among E. coli.
AB - The genomes of a diverse set of Escherichia coli, including many Shiga toxin-producing strains of various serotypes were determined. A total of 39 plasmids were identified among these strains, and many carried virulence or putative virulence genes of Shiga toxin-producing E. coli strains, virulence genes for other pathogenic E. coli groups, and some had combinations of these genes. Among the novel plasmids identified were eight that carried resistance genes to aminoglycosides, carbapenems, penicillins, cephalosporins, chloramphenicol, dihydrofolate reductase inhibitors, sulfonamides, tetracyclines and resistance to heavy metals. Two of the plasmids carried six of these resistance genes and two novel IncHI2 plasmids were also identified. The results of this study showed that plasmids carrying diverse resistance and virulence genes of various pathogenic E. coli groups can be found in E. coli strains and serotypes regardless of the isolate's source and therefore, is consistent with the premise that these mobile elements carrying these traits may be broadly disseminated among E. coli.
KW - Antibiotic resistance
KW - Genome sequencing
KW - Plasmids
KW - STEC
KW - Virulence genes
UR - http://www.scopus.com/inward/record.url?scp=84953790378&partnerID=8YFLogxK
U2 - 10.1016/j.plasmid.2015.12.001
DO - 10.1016/j.plasmid.2015.12.001
M3 - Article
C2 - 26746359
AN - SCOPUS:84953790378
SN - 0147-619X
VL - 83
SP - 8
EP - 11
JO - Plasmid
JF - Plasmid
ER -