Printed Paper Substrates with Plasmonic and Magnetic Nanoprobes for SERS Detection of Cancer Biomarkers

Seyed Danial Mousavi; Jing Li; Justine Gordon; Han Wen Cheng; Zakiya Skeete; Nina Filippone; Lindsey Walter; Sydney Hakimi; Melissa Hader; Hallie Feldman; Tony Yuan; Chuan Jian Zhong

doi:10.1021/acsanm.5c03158

Printed Paper Substrates with Plasmonic and Magnetic Nanoprobes for SERS Detection of Cancer Biomarkers

Seyed Danial Mousavi, Jing Li, Justine Gordon, Han Wen Cheng^*, Zakiya Skeete, Nina Filippone, Lindsey Walter, Sydney Hakimi, Melissa Hader, Hallie Feldman, Tony Yuan, Chuan Jian Zhong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Sensitivity and multiplexing capability are essential for developing effective cancer-screening biosensors. This is addressed in this work by coupling plasmonic and magnetic nanoprobes with wax-printed fibrous substrates in direct or microfluidic configurations using a portable Raman spectrometer. Gold nanoparticles and nickel–iron alloyed magnetic cores with gold shells, upon bioconjugation and Raman labeling, are exploited as nanoprobes for the surface-enhanced Raman scattering (SERS) detection of cancer biomarkers. The enrichment of the nanoprobe “hot spots” within the wax-confined fibrous space enables enhanced detection of the targeted biomarkers, such as carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE). The detection is also enhanced by magnetic focusing using plasmonic-magnetic nanoprobes. In the case of wax-printed fibrous substrates, the confined conjugated plasmonic nanoprobes allowed for enhancement of the detection. In the case of magnetic-plasmonic nanoprobes, microfluidic magnetic focusing facilitated effective detection. Both plasmonic and plasmonic-magnetic nanoparticles have successfully detected CEA and NSE, demonstrating the viability of multiplexing using distinct Raman labels. These findings have significant implications for developing effective SERS-based early cancer detection strategies.

Original language	English
Pages (from-to)	15395-15404
Number of pages	10
Journal	ACS Applied Nano Materials
Volume	8
Issue number	30
DOIs	https://doi.org/10.1021/acsanm.5c03158
State	Published - 1 Aug 2025

Keywords

fibrous paper
magnetic focusing
microfluidic
multiplexing
plasmonic nanoprobes
SERS detection

Access to Document

10.1021/acsanm.5c03158

Cite this

@article{82d449d674d64945ae303a42c90b9ba7,

title = "Printed Paper Substrates with Plasmonic and Magnetic Nanoprobes for SERS Detection of Cancer Biomarkers",

abstract = "Sensitivity and multiplexing capability are essential for developing effective cancer-screening biosensors. This is addressed in this work by coupling plasmonic and magnetic nanoprobes with wax-printed fibrous substrates in direct or microfluidic configurations using a portable Raman spectrometer. Gold nanoparticles and nickel–iron alloyed magnetic cores with gold shells, upon bioconjugation and Raman labeling, are exploited as nanoprobes for the surface-enhanced Raman scattering (SERS) detection of cancer biomarkers. The enrichment of the nanoprobe “hot spots” within the wax-confined fibrous space enables enhanced detection of the targeted biomarkers, such as carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE). The detection is also enhanced by magnetic focusing using plasmonic-magnetic nanoprobes. In the case of wax-printed fibrous substrates, the confined conjugated plasmonic nanoprobes allowed for enhancement of the detection. In the case of magnetic-plasmonic nanoprobes, microfluidic magnetic focusing facilitated effective detection. Both plasmonic and plasmonic-magnetic nanoparticles have successfully detected CEA and NSE, demonstrating the viability of multiplexing using distinct Raman labels. These findings have significant implications for developing effective SERS-based early cancer detection strategies.",

keywords = "fibrous paper, magnetic focusing, microfluidic, multiplexing, plasmonic nanoprobes, SERS detection",

author = "Mousavi, {Seyed Danial} and Jing Li and Justine Gordon and Cheng, {Han Wen} and Zakiya Skeete and Nina Filippone and Lindsey Walter and Sydney Hakimi and Melissa Hader and Hallie Feldman and Tony Yuan and Zhong, {Chuan Jian}",

note = "Publisher Copyright: {\textcopyright} 2025 American Chemical Society",

year = "2025",

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doi = "10.1021/acsanm.5c03158",

language = "English",

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T1 - Printed Paper Substrates with Plasmonic and Magnetic Nanoprobes for SERS Detection of Cancer Biomarkers

AU - Mousavi, Seyed Danial

AU - Li, Jing

AU - Gordon, Justine

AU - Cheng, Han Wen

AU - Skeete, Zakiya

AU - Filippone, Nina

AU - Walter, Lindsey

AU - Hakimi, Sydney

AU - Hader, Melissa

AU - Feldman, Hallie

AU - Yuan, Tony

AU - Zhong, Chuan Jian

PY - 2025/8/1

Y1 - 2025/8/1

N2 - Sensitivity and multiplexing capability are essential for developing effective cancer-screening biosensors. This is addressed in this work by coupling plasmonic and magnetic nanoprobes with wax-printed fibrous substrates in direct or microfluidic configurations using a portable Raman spectrometer. Gold nanoparticles and nickel–iron alloyed magnetic cores with gold shells, upon bioconjugation and Raman labeling, are exploited as nanoprobes for the surface-enhanced Raman scattering (SERS) detection of cancer biomarkers. The enrichment of the nanoprobe “hot spots” within the wax-confined fibrous space enables enhanced detection of the targeted biomarkers, such as carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE). The detection is also enhanced by magnetic focusing using plasmonic-magnetic nanoprobes. In the case of wax-printed fibrous substrates, the confined conjugated plasmonic nanoprobes allowed for enhancement of the detection. In the case of magnetic-plasmonic nanoprobes, microfluidic magnetic focusing facilitated effective detection. Both plasmonic and plasmonic-magnetic nanoparticles have successfully detected CEA and NSE, demonstrating the viability of multiplexing using distinct Raman labels. These findings have significant implications for developing effective SERS-based early cancer detection strategies.

AB - Sensitivity and multiplexing capability are essential for developing effective cancer-screening biosensors. This is addressed in this work by coupling plasmonic and magnetic nanoprobes with wax-printed fibrous substrates in direct or microfluidic configurations using a portable Raman spectrometer. Gold nanoparticles and nickel–iron alloyed magnetic cores with gold shells, upon bioconjugation and Raman labeling, are exploited as nanoprobes for the surface-enhanced Raman scattering (SERS) detection of cancer biomarkers. The enrichment of the nanoprobe “hot spots” within the wax-confined fibrous space enables enhanced detection of the targeted biomarkers, such as carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE). The detection is also enhanced by magnetic focusing using plasmonic-magnetic nanoprobes. In the case of wax-printed fibrous substrates, the confined conjugated plasmonic nanoprobes allowed for enhancement of the detection. In the case of magnetic-plasmonic nanoprobes, microfluidic magnetic focusing facilitated effective detection. Both plasmonic and plasmonic-magnetic nanoparticles have successfully detected CEA and NSE, demonstrating the viability of multiplexing using distinct Raman labels. These findings have significant implications for developing effective SERS-based early cancer detection strategies.

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